Purchase of a Fast Protein Liquid Chromatography System

该提案要求获得支持以获得快速蛋白质液相色谱(FPLC)系统。该仪器将由几名研究人员共享，他们的研究工作需要分离和纯化蛋白质或寡核苷酸。提出了以下研究建议。核苷酸切除DNA修复的生化机制，金属的结构和催化机制，细胞色素c的结构和内部动力学，ubiluitin和钙调蛋白的功能，植物系统中蛋白质的生物合成和细胞生长的调节，核酸的构象及其与小分子的相互作用，染色体蛋白质磷酸化在染色体结构、细胞分裂和胚胎发育中的作用。

This proposal requests support to obtain a Fast Protein Liquid Chromatography (FPLC) system. The instrument will be shared by several investigators whose research efforts require the separation and purification of proteins or oligonucleotide. The following studies are proposed. Biochemical mechanisms of nucleotide excision DNA repair, structure and catalytic mechanisms of metal.activated enzymes, structure and internal dynamics in cytochrome c, ubiguitin and calmodulin function, protein biosynthesis and the regulation of cell growth in plant systems, conformations of nucleic acids and their interactions with small molecules, the role of chromosomal proteins phosphorylation in chromosomal structure, cell division and embryonic development.