Purchase of a Fast Protein Liquid Chromatography System

购买快速蛋白质液相色谱系统

• 批准号: 8812108
• 负责人: Anthony Yeung
• 金额: $ 3万
• 依托单位: Institute For Cancer Research
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1988
• 资助国家: 美国
• 起止时间: 1988-11-15 至 1991-04-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8812108&HistoricalAwards=false
• 关键词: Purchase; Fast; Protein; Liquid; Chromatography

This proposal requests support to obtain a Fast Protein Liquid Chromatography (FPLC) system. The instrument will be shared by several investigators whose research efforts require the separation and purification of proteins or oligonucleotide. The following studies are proposed. Biochemical mechanisms of nucleotide excision DNA repair, structure and catalytic mechanisms of metal.activated enzymes, structure and internal dynamics in cytochrome c, ubiguitin and calmodulin function, protein biosynthesis and the regulation of cell growth in plant systems, conformations of nucleic acids and their interactions with small molecules, the role of chromosomal proteins phosphorylation in chromosomal structure, cell division and embryonic development.

该提案请求支持以获得快速蛋白质液相色谱 (FPLC) 系统。 该仪器将由几位研究人员共享，他们的研究工作需要分离和纯化蛋白质或寡核苷酸。 建议进行以下研究。 核苷酸切除的生化机制DNA修复、金属激活酶的结构和催化机制、细胞色素c的结构和内部动力学、泛素和钙调蛋白功能、植物系统中蛋白质生物合成和细胞生长的调节、核酸的构象及其与小分子的相互作用、染色体蛋白质磷酸化的作用 染色体结构、细胞分裂和胚胎发育。