Vesicle-Microtubule Interaction and Fast Axonal Transport

这项工作的重点是表征重要的运动相关的 与膜结合囊泡表面相关的蛋白质和/或 与微管的亚单位晶格结合。 的亚细胞分布 这些蛋白质，称为驱动蛋白和vesikin，将在 光学和电子显微镜水平以及蛋白质的身份 将确定与vesikin或驱动蛋白特异性相关 通过亲和层析。 这两种方法都将利用 标准生化和免疫技术。 以比较的 方法，线粒体将被分析，以确定 这种细胞器的特性使其能够双向移动， 轴突 这一点很重要，因为到目前为止， 专注于轴突中单向运动的轴浆囊泡。 最后，选择驱动蛋白和vesikin的cDNA克隆， 研究了 这些实验的重要性在于它们将增加我们的 了解参与重要细胞的马达分子 细胞内颗粒运动的生物学现象， 尤其是快速轴突运输。

The work focuses on the characterization of important motility related proteins associated with the surface of membrane bound vesicles and/or with the subunit lattice of microtubules. The subcellular distribution of these proteins, called kinesin and vesikin, will be determined at the light and electron microscopic levels, and the identity of proteins that associate specifically with vesikin or kinesin will be determined by affinity chromatography. Both of these approaches will utilize standard biochemical and immunological techniques. In a comparative approach, mitochondria will be analyzed to determine what characteristics of that organelle allow it to move bidirectionally in axons. This is important because up to now motility studies have focused on axoplasmic vesicles that move unidirectionally in axons. Finally, cDNA clones of kinesin and vesikin will be selected and studied. The significance of these experiments is that they will increase our understanding of the motor molecules involved in the important cell biological phenomena of intracellular particle motility in general and fast axonal transport in particular.