Vesicle-Microtubule Interaction and Fast Axonal Transport

囊泡-微管相互作用和快速轴突运输

• 批准号: 8820553
• 负责人: Roger Sloboda
• 金额: $ 23.84万
• 依托单位: Dartmouth College
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1989
• 资助国家: 美国
• 起止时间: 1989-09-01 至 1992-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8820553&HistoricalAwards=false
• 关键词: Vesicle; Microtubule; Interaction; Fast; Axonal

The energy-driven movement of intracellular membrane-bounded particles from one region of the cell to another is mediated by translocator molecules which transport the particles along microtubules. In axons, retrograde movement is mediated by a protein called "cytoplasmic dynein," while anterograde movement is mediated by a different protein called "kinesin." In this project, the subcellular distribution of kinesin and cytoplasmic dynein will be determined by immunolocalization at the light and electron microscopic levels. The differential association of these proteins with vesicles moving in the orthograde vs. retrograde directions in axons will be assessed. The components of the outer mitochondrial membrane will be analyzed to determine the characteristics of that organelle which allow it to move bidirectionally in axons. Finally, cDNA clones of the genes coding for the translocator proteins will be isolated. These experiments have as their focus increasing our understanding of the motor molecules involved in the important cell biological phenomenon of intracellular particle motility.

能量驱动的细胞内膜结合运动 从细胞的一个区域到另一个区域是由 转运分子，其将颗粒沿着 微管 在轴突中，逆行运动是由一个 蛋白质称为“细胞质动力蛋白”，而顺行运动 是由一种叫做“驱动蛋白”的不同蛋白质介导的。“在这个 项目，驱动蛋白和细胞质的亚细胞分布 动力蛋白将通过在光下的免疫定位来确定， 电子显微镜水平。 的差异关联 这些蛋白质与囊泡移动在顺行与 将评估轴突中的逆行方向。 的组件 将分析线粒体外膜的厚度以确定 细胞器的特性使其能够移动 在轴突中是双向的。 最后，基因的cDNA克隆 编码转运蛋白的基因将被分离。 这些实验的重点是增加我们的 了解参与重要的 细胞内粒子运动的细胞生物学现象。