Engineering Approaches to Enhancing Secondary Metabolite Production in Plant Tissue Culture

本研究将利用两阶段植物组织培养系统， 研究各种环境因素对控制 次级代谢产物的产生。 为此选择的模型系统 目的是生产迷迭香酸（抗水肿药物）， 种鞘蕊花和/或鼠尾草。环境 待研究的因素包括溶解氧/二氧化碳， 剪切和质量传递。 后两个因素涉及 搅拌和细胞聚集对次级代谢产物的影响 生产 这项研究的重要性是双重的。 首先，它不仅是 有助于工程师获得工厂的放大数据 悬浮细胞系统具有简单的模型系统， 工作，而且植物生理学家和遗传学家更喜欢植物 悬浮培养，而不是大田作物， 他们的实验。 第二，在使用和扩展方面的经验 植物细胞系统，用于生产重要的化学品， 随着人口增长及其相关的 住房需求占用了农业生产用地。 植物细胞在生物反应器中生长，可以在三分之一的时间内扩增 与大田作物只有两个维度相比， 替代生产手段，特别是贵重药品的替代生产手段。

This study will utilize a two stage plant tissue culture system to study the effect of various environmental factors on the control of secondary metabolite production. The model system chosen for this purpose is the production of rosmarinic acid (an antiedema drug) by species of Coleus blumei and/or Salvia officinalis. The environmental factors to be investigated include dissolved oxygen/carbondioxide, shear, and mass transfer. These latter two factors involve the effects of agitation and cell aggregation on secondary metabolite production. The importance of this study is two-fold. First, not only will it be useful to the engineer in obtaining scale-up data for plant suspension cell systems to have a simple model system with which to work, but also the plant physiologist and geneticist prefer plant suspension cultures rather than field crops in which to carry out their experiments. Secondly, experience in working with and scaling up plant cell systems for the production of important chemicals and drugs will become necessary as population growth and its associated housing needs encroaches on land use for agriculture production. Plant cell growth in bioreactors, which can expand in a third dimension compared with only two dimensions for field crops, offers an alternative means of production, particularly of valuable drugs.