Membrane-Associated Phosphorylation

本项目探讨亚硫酸盐替代光激活预还原或胰蛋白酶处理的菠菜类囊体的潜在atp酶的可能机制。亚硫酸盐可能阻止紧密结合的ADP和镁离子的抑制作用。研究了亚硫酸盐对Mg2+和ADP在不同通电水平和不同类囊体活化状态下的抑制动力学。减少的CF1，在35%甲醇中通过MgATP水解耗尽的腺苷酸应该是永久活性的。初始反应将通过停止流动技术进行研究，以观察亚硫酸盐或腺苷酸耗尽是否消除了滞后。詹根多夫博士做了一个有趣的观察，亚硫酸盐可以代替光在光合作用中产生能量。他现在正利用这一效应来研究叶绿体中能量产生的基本机制。这一过程对地球上的生命至关重要，因为它将光能转化为化学能，以生产碳水化合物

This project explores possible mechanisms in the sulfite replacement of light in activating latent ATPase of pre- reduced or trypsin treated spinach thylakoids. Sulfite might prevent inhibition by tightly bound ADP and magnesium ions. Kinetics of Mg2+ and ADP inhibition are being investigated at different levels of energization, and with different states of thylakoid activation using sulfite. Reduced CF1, depleted of adenylates by MgATP hydrolysis in 35% methanol should be permanently active. Initial reactions will be studied by stop-flow technique, to see if lags are eliminated by sulfite or by adenylate depletion. Dr. Jagendorf has made the interesting observation that sulfite can substitute for light leading to energy generation during photosynthesis. He is now using this effect to study the basic mechanism of energy generation in chloroplasts. This process is of utmost importance to life on earth as it converts light to chemical energy for the production of carbohydrates.***//