Membrane-Associated Phosphorylation

本研究探讨了亚硫酸盐替代光照激活预还原或胰蛋白酶处理的菠菜类囊体的潜在ATP酶的可能机制。亚硫酸盐可能会阻止紧密结合的ADP和镁离子的抑制作用。Mg2+和ADP抑制的动力学正在研究在不同水平的亚硫酸盐，并与不同状态的类囊体激活。通过在35%甲醇中的MgATP水解耗尽腺苷酸的还原CF 1应该是永久活性的。将通过停流技术研究初始反应，以观察是否通过亚硫酸盐或腺苷酸消耗消除滞后。约克夏夫博士做了一个有趣的观察，即亚硫酸盐可以代替光，从而在光合作用中产生能量。他现在正在利用这种效应来研究叶绿体中能量产生的基本机制。这一过程对地球上的生命至关重要，因为它将光转化为化学能以产生碳水化合物。

This project explores possible mechanisms in the sulfite replacement of light in activating latent ATPase of pre- reduced or trypsin treated spinach thylakoids. Sulfite might prevent inhibition by tightly bound ADP and magnesium ions. Kinetics of Mg2+ and ADP inhibition are being investigated at different levels of energization, and with different states of thylakoid activation using sulfite. Reduced CF1, depleted of adenylates by MgATP hydrolysis in 35% methanol should be permanently active. Initial reactions will be studied by stop-flow technique, to see if lags are eliminated by sulfite or by adenylate depletion. Dr. Jagendorf has made the interesting observation that sulfite can substitute for light leading to energy generation during photosynthesis. He is now using this effect to study the basic mechanism of energy generation in chloroplasts. This process is of utmost importance to life on earth as it converts light to chemical energy for the production of carbohydrates.***//