Structure of Microbial Communities Through 16S rRNA Analysis: cDNA and Hybridization Probe Methods

通过 16S rRNA 分析微生物群落结构：cDNA 和杂交探针方法

• 批准号: 8818358
• 负责人: David Ward
• 金额: $ 6万
• 依托单位: Montana State University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1989
• 资助国家: 美国
• 起止时间: 1989-01-01 至 1990-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8818358&HistoricalAwards=false
• 关键词: Structure; Microbial; Communities; Through; 16S

Microbial ecologists are unsure of the composition and structure of any microbial community, because traditional methods cannot ensure the recognition of all community members. This provides a poor basis for understanding how toxicants and genetically engineered organisms affect natural microbial communities. New solutions to this problem come from molecular phylogeny and improvements in recombinant-DNA technology. 16S rRNA is a unique cellular component useful for describing microbial communities. Prior work on this approach leads to the proposed development of a method for retrieving 16S rRNA sequence information from natural communities as copy DNA, valuable in that it permits quantitative as well as qualitative analysis of community members. The limitations and scope of the method will be analyzed on a hot spring cyanobacterial mat community as a model.

微生物生态学家不确定其组成， 任何微生物群落的结构，因为传统的方法 不能确保所有社区成员的认可。 这 为理解有毒物质和 基因工程生物影响自然微生物 社区. 这个问题的新解决方案来自分子 重组DNA技术的发展和改进。 16S rRNA是一种独特的细胞成分，可用于描述 微生物群落 关于这种方法的先前工作导致了 提出了一种检索16S rRNA序列的方法 来自自然界的DNA拷贝信息， 它允许定量和定性分析， 社区成员。 该方法的局限性和范围将 作为一个温泉蓝藻垫群落分析， 模型