Analysis of Microbial Community Structure Using 16S rRNA- Based Methods

使用基于 16S rRNA 的方法分析微生物群落结构

• 批准号: 8907611
• 负责人: David Ward
• 金额: $ 30.73万
• 依托单位: Montana State University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1989
• 资助国家: 美国
• 起止时间: 1989-07-15 至 1992-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8907611&HistoricalAwards=false
• 关键词: Analysis; Microbial; Community; Structure; Using

Microbial ecologist are unsure of the composition and Traditional culture dependent methods for microbial communities cannot ensure the recognition of all community members. This limits understanding of how toxicants and genetically engineered organisms affect natural microbial communities. Recently the development of new molecular methods for analyzing a specific cellualr component, 16S ribosomal RNA, was completed as a means of identifying the various inhabitants of natural microbial communities without traditional culture techniques. The initial results using these methods demonstrate that natural communities do contain many inhabitants which were previously not noted in microbial studies. The composition and structure of a model hot spring cyanobacterial mat community will be determined in this proposal through in depth comparative analysis of 16S rRNA sequences previously obtained for the microbial mat. This should reveal the magnitude of the differences between real community complexity and that portion described by the pure culture collection. The well-studied nature of this model will also be explpoited to investigate the trophic structure of the community, as well as possible problems limiting the application of all rRNA methods usng 16S rRNA-based hybridization probes.

微生物生态学家不确定其组成， 微生物群落的传统培养依赖方法 不能确保所有社区成员的认可。 这 限制了对有毒物质和基因工程如何 生物体影响天然微生物群落。 最近 开发新的分子方法来分析特定的 细胞组分16S核糖体RNA作为一种手段， 识别自然微生物的各种居民 没有传统文化技术的社区。 初始 结果表明，自然群落 有许多以前没有注意到的居民， 微生物研究 模型热的组成和结构 春季蓝藻垫群落将在此确定 通过16S rRNA的深入比较分析， 先前获得的微生物垫的序列。 这应该 揭示了真实的社区和 复杂性和纯培养所描述的部分 收藏. 这个模型的充分研究的性质也将是 对群落的营养结构进行了调查， 以及限制所有rRNA应用的可能问题 方法采用基于16S rRNA的杂交探针。