Ecology of a Model Hot Spring Microbial Community: Relevance of Cultivated and Uncultivated Species

温泉微生物群落模型的生态学：栽培和未栽培物种的相关性

• 批准号: 9209677
• 负责人: David Ward
• 金额: $ 46万
• 依托单位: Montana State University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1992
• 资助国家: 美国
• 起止时间: 1992-09-01 至 1999-02-28
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9209677&HistoricalAwards=false
• 关键词: Ecology; Model; Hot; Spring; Microbial

This proposal addresses a fundamental problem limiting progress in microbial ecology--the poor correlation between cultivated and naturally occurring microorganisms. This paradox has been revealed using small-subunit ribosomal RNA (SSU rRNA) sequences to characterize a model hot spring cyanobacterial mat community. Because the isolates come mainly from the model community, the model is particularly well-suited for investigating the basis for this discrepancy. rRNA-based hybridization probe studies of cultivated and uncultivated phototrophic microorganisms of the mat provide a more focused view of the boundaries of the model community, and permit formulation of several hypotheses that are likely to explain the failure to detect SSU rRNAs of cultivated species. Hypotheses are based on how trophic structure affects abundance and how culture procedures select for microorganisms not adapted hypotheses, (a) a more streamlined search for SSU rRNAs of natural community members, (b) a comprehensive probe study to determine the distributions and numeric relevance of all cultivated and uncultivated species, and (c) the cultivation of more relevant species using more ecologically sound approaches will be undertaken. The results are essential for future studies as well as parallel studies of the mat as a model for ancient microbial communities.

这一建议解决了一个限制进展的根本问题 在 微生物生态学-栽培和 自然发生的微生物 这个悖论一直是 透露 使用小亚基核糖体RNA（SSU rRNA）序列， 描述了一个典型的温泉蓝藻垫群落。 由于分离株主要来自模式群体， 模型是特别适合调查的基础， 这个矛盾。 基于rRNA的杂交探针研究 培养的和未培养的光养微生物 垫 提供模型边界的更集中视图 社区，并允许制定几个假设， 这可能解释了未能检测到SSU rRNA的培养， 物种 假设是基于营养结构如何影响 丰度和培养程序如何选择微生物 不 适应假设，（a）更精简的SSU rRNA搜索 的 自然群落成员，（B）一个全面的探索性研究， 确定所有的分布和数字相关性 栽培 （c）种植更多的植物; 相关 使用更生态无害的方法的物种将 进行。 这些结果对未来的研究也是必不可少的 作为古代微生物模型的平行研究 社区.