Redefining the immune landscape of the human ocular surface

重新定义人类眼表的免疫景观

• 批准号: DP230102105
• 负责人: Prof Laura Downie
• 金额: $ 46.59万
• 依托单位: The University of Melbourne
• 依托单位国家: 澳大利亚
• 项目类别: Discovery Projects
• 财政年份: 2023
• 资助国家: 澳大利亚
• 起止时间: 2023-04-03 至 2026-04-02
• 项目状态: 未结题
• 来源: https://dataportal.arc.gov.au/RGS/Web/Grants/DP230102105
• 关键词: Redefining; immune; landscape; human; ocular

At the ocular surface, the cornea and limbus need to mount effective immune responses to maintain corneal transparency for clear vision. The current paradigm is that the human cornea houses the same innate immune cell subsets (dendritic cells and macrophages) as naïve mice in pathogen-free facilities. Our pilot data challenge this premise, with early evidence that innate and adaptive cells (T cells) coexist in normal human corneas. Integrating state-of-the-art techniques, we will advance understanding of immune regulation at the human ocular surface by comprehensively defining immune cell biology and dynamics. We will define the effect of age on immune cells in these tissues, and relationships between the tear proteome and cell behaviours.

在眼表面，角膜和利姆布斯需要产生有效的免疫应答以维持角膜透明度以获得清晰的视觉。目前的范例是，人类角膜容纳了与无病原体设施中的幼稚小鼠相同的先天免疫细胞亚群（树突状细胞和巨噬细胞）。我们的试验数据挑战了这一前提，早期证据表明先天和适应性细胞（T细胞）共存于正常人类角膜中。结合最先进的技术，我们将通过全面定义免疫细胞生物学和动力学来促进对人类眼表免疫调节的理解。我们将确定年龄对这些组织中免疫细胞的影响，以及泪液蛋白质组和细胞行为之间的关系。