PYI: Ecology and Genetics of Microbial Evolution

PYI：微生物进化的生态学和遗传学

• 批准号: 8858820
• 负责人: Richard Lenski
• 金额: $ 17.45万
• 依托单位: University of California-Irvine
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1988
• 资助国家: 美国
• 起止时间: 1988-07-15 至 1992-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8858820&HistoricalAwards=false
• 关键词: PYI; Ecology; Genetics; Microbial; Evolution

Funding from the prestigious five-year Presidential Young Investigator award will be used to support research on the population biology of bacteria and their plasmids. Their large populations and short generations permit an experimental, not merely correlative, approach to the study of evolutionary processes. Research efforts will fall into four major areas: genetic basis of fitness in evolving populations of Escherichia coli, fitness consequences associated with alternative modes of regulation of tetracycline resistance genes in E. coli, evolution and genetics of an association between E. coli and plasmid pACYC184, and effects of recombinant functions (particularly chlorobenzoate degradation) on plasmid stability and host fitness in Pseudomonas species. Precise measurements of the effects of particular genetic variants, both chromosomal and extrachromosomal, on bacterial net growth, or relative fitness, are required for these projects.

享有盛誉的五年期​​总统青年研究员奖的资金将用于支持细菌及其质粒的群体生物学研究。 它们的种群数量大、世代短，因此可以采用实验性的、而不仅仅是相关性的方法来研究进化过程。 研究工作将分为四个主要领域：大肠杆菌进化种群适应性的遗传基础、与大肠杆菌中四环素抗性基因的替代调节模式相关的适应性后果、大肠杆菌和质粒 pACYC184 之间关联的进化和遗传学，以及重组功能（特别是氯苯甲酸酯降解）对质粒稳定性和宿主适应性的影响。 假单胞菌属物种。 这些项目需要精确测量特定遗传变异（染色体和染色体外）对细菌净生长或相对适应性的影响。