Structure and Function of the Prochlorothrix Hollandica Photosynthetic Antenna System

光合作用原核生物，Prochlorothrix hollandica， 唯一已知的自由生活的细菌， 高等植物触角色素叶绿素B。 该特征具有 发起了许多比较研究，探讨是否P。 Hollandica天线在结构和功能上类似于 与高等植物相比。 以前的其他研究 实验室已经证明类囊体膜的堆积 发生在P. hollandica中，这一功能归因于LHCII 绿色植物叶绿体的触角。 由于P. hollandica 叶绿素a/B触角结构比叶绿体简单 LHCII，本研究对中国对虾的触角蛋白进行了研究。 荷兰探讨膜的结构基础 堆叠。 实验方法结合了分子 生物、生物化学和生物物理技术， 具体问题如下：（1） 辅助叶绿素蛋白之间的进化关系？（二） 光合生物如何适应不断变化的光线 条件？及3）结构要求为何 类囊体膜堆积？% 光合作用释放氧气的光反应包括 两种膜结合的色素结合复合物， 它们参与初级光化学反应，而那些 其通过收集光来提供辅助天线功能 将能量转移到主系统。 一个伟大 最近获得了大量关于 叶绿素蛋白 相反，许多结构 天线复合体是不知道这个高水平的 分辨率 本研究的重点是考察一个班级 原核生物触角叶绿素蛋白质 理解它们的合成、组装和相互关系， 叶绿体触角系统。

The photosynthetic prokaryote, Prochlorothrix hollandica, is the only free-living bacterium known which contains the higher plant antenna pigment chlorophyll b. This feature has initiated many comparative studies examining whether the P. hollandica antenna is structurally and functionally similar to that of higher plants. Previous studies from other laboratories have shown that thylakoid membrane stacking occurs in P. hollandica, a function ascribed to the LHCII antenna of green plant chloroplasts. Since the P. hollandica Chl a/b antenna is structurally simpler than chloroplast LHCII, this research studies the antenna proteins of P. hollandica to probe the structural basis for membrane stacking. The experimental approach combines molecular biological, biochemical and biophysical techniques to address the following specific questions: 1) What are the evolutionary relationships among accessory Chl-proteins?; 2) How do photosynthetic organisms adapt to changing light conditions?; and 3) What are the structural requirements for thylakoid membrane stacking?%%% The light reactions of oxygen evolving photosynthesis involve two kinds of membrane-bound pigment-binding complexes,those which are involved in the primary photochemistry and those which serve an accessory antenna function by harvesting light energy to be transferred to the primary systems. A great deal of information has been recently obtained about the chlorophyll-proteins. In contrast, the structure of many antenna complexes is not known to this high level of resolution. The focus of this research is to examine a class of prokaryotic antenna chlorophyll-proteins to help understand their synthesis, assembly and relatedness to chloroplast antennae systems.***