Studies on the Role of Zinc and Lipids in Sperm Longevity (Biological Sciences)

锌和脂质在精子寿命中的作用研究（生物科学）

• 批准号: 8903125
• 负责人: Elizabeth Crichton
• 金额: $ 19.02万
• 依托单位: Pennsylvania State Univ University Park
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1990
• 资助国家: 美国
• 起止时间: 1990-01-01 至 1993-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8903125&HistoricalAwards=false
• 关键词: Studies; Role; Zinc; Lipids; Sperm

This research will initiate studies on sperm plasma membrane lipids and continue previous studies on the possible role of zinc in bat sperm longevity by pursuing the effect on sperm of the removal of these substances. Dr. Crichton has documented the presence of zinc in large quantities in sperm filled storage organs and she hypothesizes that long lived bat sperm may be rendered particularly resistant to prefertilization changes by inherent components that perhaps are altered at ovulation as a function of either the environmental milieu at the time, or as a programmed, time dependent result of their long stay in the female tract. In separate experiments, she will remove cholesterol and zinc from these cells in vitro, and test their viability and fertilizability by examining the status of the acrosome and by recording their metabolism and their ability to decondense within the ooplasm. The results of these studies should yield information on the role of sperm-bound lipids and zinc in sperm longevity in bats which, in turn, might answer questions on the regulatory mechanism of sperm storage. The elucidation of the mechanisms underlying sperm storage in bats holds a key to the answers of many questions in mammalian sperm biology. The possibility of controlling sperm longevity would have significant application to fertility and sterility problems in man and his agricultural animals. Interactive activities include forming a Reproductive Biology journal club, teaching a graduate course on the corpus luteum and participate in teaching an Electron Microscopy course, a course in Gamete Biology and one in Histology. In addition, Dr. Crichton will attend faculty meetings and present a seminar. This project furthers VPW program objectives which are (1) to provide opportunities for women to advance their careers in engineering and in the disciplines of science supported by NSF and (2) to encourage women to pursue careers in science and engineering by providing greater visibility for women scientists and engineers employed in industry, government, and academic institutions. By encouraging the participation of women in science, it is a valuable investment in the Nation's future scientific vitality.

本研究为精子膜脂的研究奠定了基础 并继续先前关于锌在蝙蝠中可能作用的研究， 精子寿命通过追求去除精子的影响， 这些物质。 Crichton博士记录了锌在 大量的精子充满了储存器官，她假设 长寿的蝙蝠精子可能会特别抵抗 优选化通过可能是 在排卵时改变，作为环境环境的函数， 在当时，或作为一个程序，时间依赖的结果，他们的长期 呆在女性通道里 在单独的实验中，她将去除 胆固醇和锌从这些细胞在体外，并测试他们的 通过检查顶体的状态， 通过记录他们的新陈代谢和他们的能力， 在卵质中。 这些研究的结果应该会产生 精子结合脂质和锌在精子中的作用的信息 蝙蝠的寿命，这反过来可能会回答关于 精子储存的调节机制。 的说明， 蝙蝠精子储存的基本机制是 哺乳动物精子生物学中许多问题的答案。 的可能性 控制精子寿命的方法， 人类和农业动物的生育和不育问题。 互动活动包括创办生殖生物学杂志 俱乐部，教授研究生课程的黄体和参与 在电子显微镜课程的教学中， 一个在组织学 此外，Crichton博士还将参加 会议并举行研讨会。 该项目进一步促进了VPW计划的目标，即（1）提供 为妇女提供在工程和技术领域发展职业生涯的机会 由NSF支持的科学学科和（2）鼓励妇女 通过提供更大的科学和工程专业知识， 在工业界就业的女科学家和女工程师的知名度， 政府和学术机构。 通过鼓励 妇女参与科学，这是一项宝贵的投资， 国家未来的科学活力。