Metabolic Pathway Responsible for Deuterium Enrichment in CAM Plants

CAM 植物中氘富集的代谢途径

• 批准号: 8904189
• 负责人: Leonel Sternberg
• 金额: $ 14.19万
• 依托单位: University of Miami
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1989
• 资助国家: 美国
• 起止时间: 1989-08-15 至 1992-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8904189&HistoricalAwards=false
• 关键词: Metabolic; Pathway; Responsible; Deuterium; Enrichment

The study of hydrogen isotopic fractionations during its incorporation into plant biomass may have important applications in plant biochemistry, physiology, physiological ecology and several other fields. Of particular interest in this proposal is the deuterium enrichment observed in non-exchangeable hydrogens of cellulose from CAM plants. Evidence is presented showing that biochemical reactions are responsible for this deuterium enrichment. The objective of the experiments outlined in this proposal is to determine which metabolic pathway is responsible for deuterium enrichment in CAM plants. Whole plant and tissue culture experiments are proposed here. The results of these experiments will determine whether deuterium enrichment can occur during the glycolysis-gluconeogenesis cycle found in CAM plants. An increased knowledge of the processes responsible for deuterium enrichment in CAM plants will be significant in the study of carbohydrate pools and source sink relations. More broadly, the results of this study will have important applications in the study of photosynthetic systems in relation to environmental parameters.

氢同位素与植物生物量结合过程的分馏研究在植物生物化学、生理学、生理生态学等领域具有重要的应用价值。在这个建议中特别感兴趣的是在CAM植物的纤维素的不可交换氢中观察到的氘富集。有证据表明，这种氘富集是由生化反应引起的。本提案中概述的实验目的是确定CAM植物中负责氘富集的代谢途径。本文提出了整株和组织培养实验。这些实验的结果将确定在CAM植物中发现的糖酵解-糖异生循环中是否会发生氘富集。增加对CAM植物中氘富集过程的了解将对碳水化合物库和源库关系的研究具有重要意义。更广泛地说，这项研究的结果将在与环境参数有关的光合系统的研究中具有重要的应用。