New Techniques for Affinity Precipitation of Proteins

蛋白质亲和沉淀新技术

• 批准号: 8904192
• 负责人: Peter Kilpatrick
• 金额: $ 19.53万
• 依托单位: North Carolina State University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1989
• 资助国家: 美国
• 起止时间: 1989-07-15 至 1991-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8904192&HistoricalAwards=false
• 关键词: New; Techniques; Affinity; Precipitation; Proteins

This project aims to develop two new techniques for purifying biological macromolecules such as enzymes and antibodies from complex media while attaining high selectivity, yield, and activity. The techniques belong to the class of affinity separation techniques but are distinguished by using affinity surfactants. It is proposed that the use of affinity surfactants increases effectiveness by limiting nonspecific hydrophobic interactions between different proteins that plague other protein precipitation techniques. The target proteins for the study are the oligomeric dehydrogenase enzymes and the class of IgG antibodies. Affinity surfactants will be synthesized and purified as part of the project. The surfactants will be used either directly dissolved in the aqueous protein solution or by incorporation into unilamellar vesicles. Precipitation is an important purification process in many chemical processes. It has had limited utility in biotechnology because of the nonspecific nature of hydrophobic interactions between proteins. Affinity surfactant precipitation of proteins is a promising technique to improve the usefulness of precipitation technology in downstream processing in biotechnology.

该项目旨在开发两种新的净化技术 生物大分子，如酶和抗体， 复杂的介质，同时获得高选择性、产率和 活动 这些技术属于亲和类 分离技术，但通过使用亲和性 表面活性剂 建议使用亲和力 表面活性剂通过限制非特异性 不同蛋白质之间的疏水相互作用， 困扰其他蛋白质沉淀技术。 目标 用于研究的蛋白质是寡聚脱氢酶 酶和IgG抗体的类别。 亲和表面活性剂 将作为项目的一部分进行合成和提纯。 的 表面活性剂将被使用，或者直接溶解在 蛋白质水溶液或通过掺入单层 囊泡 在许多情况下，沉淀是重要的净化过程。 化学过程。 它的效用有限， 由于疏水性的非特异性， 蛋白质之间的相互作用。 亲和表面活性剂 蛋白质沉淀是一种很有前途的技术， 降水技术在下游的作用 生物技术的加工。