Flagellar Glycoprotein Dynamics and Whole Cell Locomotion

鞭毛糖蛋白动力学和全细胞运动

• 批准号: 8905530
• 负责人: Robert Bloodgood
• 金额: $ 26.1万
• 依托单位: University of Virginia Main Campus
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1989
• 资助国家: 美国
• 起止时间: 1989-09-01 至 1993-02-28
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8905530&HistoricalAwards=false
• 关键词: Flagellar; Glycoprotein; Dynamics; Whole; Cell

The goal of this research is to understand the mechanism involved in force transduction occurring at the cell surface that is responsible for whole cell locomotion. The movement of flagellar membrane glycoproteins (in particular, a pair of 350 kDa concanavalin A-binding glycoproteins) within the plane of the flagellar membrane are involved in the whole cell gliding locomotion exhibited by the eukaryotic algal cell Chlamydomonas. Crosslinking of the flagellar membrane glycoproteins triggers a transmembrane signaling pathway that activates intraflagellar machinery responsible for movement of the 350 kDa glycoproteins within the plane of the flagellar membrane. Experiments are proposed to analyze the roles of calcium, cAMP, cGMP and protein phosphorylation in the transmembrane signaling pathway in the flagellum. It will be determined whether crosslinking of flagellar surface glycoproteins is necessary to activate the transmembrane signaling pathway and whether the crosslinking results in a tighter association of flagellar membrane glycoproteins with the underlying cytoskeleton (axoneme). Experiments are proposed to develop an in vitro model system for reactivating flagellar surface motility and to exploit such a system to understand the transmembrane signaling the generation of force for glycoprotein movement. Efforts will be made to identify and characterize the components of the motor responsible for flagellar membrane glycoprotein dynamics and gliding motility. Understanding the mechanisms by which cells control the distribution of plasma membrane proteins and by which they actively change the distribution of these proteins will have significance for understanding the functioning of higher organisms as well as unicellular algae.

这项研究的目的是了解所涉及的机制 在细胞表面发生的力传递中， for whole整个cell细胞movement运动. 鞭毛膜的运动 糖蛋白（特别是一对350 kDa伴刀豆球蛋白A结合蛋白） 鞭毛膜平面内的糖蛋白）参与 在真核藻类的整个细胞滑动运动中， 细胞衣原体 鞭毛膜的交联 糖蛋白触发跨膜信号通路， 鞭毛内机器负责运动的350 kDa 鞭毛膜平面内的糖蛋白。 实验 分析了钙离子、cAMP、cGMP和蛋白质的作用 鞭毛中跨膜信号通路的磷酸化。 将确定鞭毛表面的交联是否 糖蛋白是激活跨膜信号传导所必需的 途径以及交联是否导致 鞭毛膜糖蛋白及其下面的细胞骨架 （轴丝）。 本实验旨在建立一个体外模型系统 用于重新激活鞭毛表面运动性，并利用这种系统， 了解跨膜信号的产生， 糖蛋白运动 将努力查明和 表征负责鞭毛的马达的组件 膜糖蛋白动力学和滑动运动。 了解细胞控制细胞的机制 分布的质膜蛋白质，并通过它们积极 改变这些蛋白质的分布， 了解高等生物的功能， 单细胞藻类