Proposal for a Table Top Ultracentrifuge Facility

关于台式超速离心机设施的提案

• 批准号: 9115828
• 负责人: Robert Bloodgood
• 金额: $ 2.68万
• 依托单位: University of Virginia Main Campus
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1992
• 资助国家: 美国
• 起止时间: 1992-08-01 至 1994-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9115828&HistoricalAwards=false
• 关键词: Proposal; Table; Top; Ultracentrifuge; Facility

Funds are requested for the purchase of a Model TLX-120 Table-Top Ultracentrifuge and Rotors from Beckman Instruments Inc. This instrument will provide new investigative capacity in the Department of Anatomy and Cell Biology and will be an important educational resource in the training of future independent investigators in contemporary molecular, cell and developmental biology. Specifically, it will make possible a variety of studies that must be conducted on very small amounts of biological materials (cells, constituent macromolecules and macromolecular assemblies) under conditions that minimize preparation time and maintain temperatures well below the ambient. Example applications by the major users include analyses of: a) protein phosphorylation as an intracellular signal in flagellar motility: b) secretion granule function in cell-free assays; c) interaction of extracellular matrix components with purified and reconstituted integrins; d) kinesin/dynein interactions with microtubules and membrane- bounded organelles; and e) stage-specific RNAs isolated from embryonic tissues. Comparable instrumentation is not available for routine use by the identified major an minor users.

请拨资金用于购买TLX-120型桌面 Beckman Instruments Inc.的超离心泵和转子。 这 该工具将为联合国提供新的调查能力， 解剖学和细胞生物学系，将是一个重要的 未来独立人才培养中的教育资源 当代分子、细胞和发育研究者 生物学 具体而言，它将使各种 研究必须在非常少量的 生物材料（细胞、组成大分子和 大分子组件）的条件下， 准备时间和保持温度远低于 周围的 主要用户的示例应用包括 分析：a）蛋白磷酸化作为细胞内 鞭毛运动信号：B）分泌颗粒在 无细胞测定; c）细胞外基质的相互作用 具有纯化和重构的整联蛋白的组分; d） 驱动蛋白/动力蛋白与微管和膜的相互作用- 结合的细胞器;和e）分离自 胚胎组织 没有可比工具可用 供所识别的主要用户和次要用户日常使用。