Proposal for a Table Top Ultracentrifuge Facility

所要求的经费用于从贝克曼仪器公司购买TLX-120型台式超离心机和转子。该仪器将为解剖和细胞生物学部门提供新的研究能力，并将成为培训当代分子、细胞和发育生物学领域未来独立研究人员的重要教育资源。具体来说，它将使必须在最小化制备时间和保持远低于环境温度的条件下对非常少量的生物材料（细胞，组成大分子和大分子组件）进行的各种研究成为可能。主要用户的示例应用包括：a)鞭毛运动中作为细胞内信号的蛋白质磷酸化分析；b)无细胞测定中的分泌颗粒功能分析；C)细胞外基质成分与纯化和重组整合素的相互作用；D)动力蛋白/动力蛋白与微管和膜结合细胞器的相互作用；e)从胚胎组织中分离的阶段特异性rna。可比较的仪器无法用于确定的主要和次要用户的日常使用。

Funds are requested for the purchase of a Model TLX-120 Table-Top Ultracentrifuge and Rotors from Beckman Instruments Inc. This instrument will provide new investigative capacity in the Department of Anatomy and Cell Biology and will be an important educational resource in the training of future independent investigators in contemporary molecular, cell and developmental biology. Specifically, it will make possible a variety of studies that must be conducted on very small amounts of biological materials (cells, constituent macromolecules and macromolecular assemblies) under conditions that minimize preparation time and maintain temperatures well below the ambient. Example applications by the major users include analyses of: a) protein phosphorylation as an intracellular signal in flagellar motility: b) secretion granule function in cell-free assays; c) interaction of extracellular matrix components with purified and reconstituted integrins; d) kinesin/dynein interactions with microtubules and membrane- bounded organelles; and e) stage-specific RNAs isolated from embryonic tissues. Comparable instrumentation is not available for routine use by the identified major an minor users.