Dynamic Light Scattering Studies of Actine Gelation

光动胶凝的动态光散射研究

• 批准号: 8905906
• 负责人: Jay Newman
• 金额: $ 15.5万
• 依托单位: Union College
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1989
• 资助国家: 美国
• 起止时间: 1989-09-01 至 1993-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8905906&HistoricalAwards=false
• 关键词: Dynamic; Light; Scattering; Studies; Actine

The process of gelation of filamentous actin and the role of actin binding proteins will be investigated under a variety of conditions using non-perturbative dynamic light scattering and viscoelastic measurements. Recent measurements at low actin concentrations using microsphere diffusion probes will be extended to study the network pore sizes and interactions within actin gels under conditions designed to mimic the cellular cytoplasm. Additional experiments will be conducted in order to clarify the role of actin in maintaining and controlling the mechanical properties of the cytoplasm of non-muscle cells. It is generally recognized that the protein actin is one of the most common proteins in nature and is present in most, if not all, eukaryotic cells. Its form ranges from the thin filament in muscle cells to mixtures of the globular monomeric form (G-actin) and polymerized actin in non-muscle cells. Recent progress has begun to reveal the role of actin in determining the structure and mechanical properties of the cytoplasmic matrix of most non-muscle cells. Actin's function in cellular processes hinges on its ability to reversibly polymerize as well as to form a viscoelastic gel, often in conjunction with specific proteins present in the cytoplasm. The mechanisms by which these various actin- binding proteins control the polymerization-depolymerization and sol-gel-sol transformations of actin are not yet well understood.

丝状肌动蛋白的凝胶化过程及 肌动蛋白结合蛋白将在各种条件下进行研究 非微扰动态光散射 和粘弹性测量。 最近的测量值低 使用微球扩散探针的肌动蛋白浓度将 可以扩展到研究网络孔径和相互作用 在肌动蛋白凝胶中， 细胞质 将进行更多的实验 为了阐明肌动蛋白在维持和 控制细胞质的机械性能， 非肌肉细胞 一般认为肌动蛋白是 是自然界中最常见的蛋白质， 不是所有的真核细胞。 它的形状从薄的 肌细胞中的细丝与球状纤维的混合物 非肌肉中的单体形式（G-肌动蛋白）和聚合肌动蛋白 细胞 最近的进展已经开始揭示肌动蛋白的作用 在确定结构和机械性能的 大多数非肌肉细胞的细胞质基质。 肌动蛋白 在细胞过程中的功能取决于它的能力， 可逆地溶胀以及形成粘弹性凝胶， 通常与存在于细胞中的特定蛋白质结合， 细胞质 这些不同的肌动蛋白- 结合蛋白控制聚合-解聚 肌动蛋白的溶胶-凝胶-溶胶转化还不是很好 明白