Engineering Research Equipment Grant: On-Line Fermentation Monitoring System

该奖项旨在提供资金，以开发一种能够监测和/或控制多个发酵参数的发酵系统。第一个项目名为“枯草芽孢杆菌新陈代谢研究”。枯草芽孢杆菌是一种重要的细菌，可以容纳克隆的外源基因和产生许多胞外蛋白。一个重要的工程问题是培养技术的发展，以实现高细胞密度和有效地将底物转化为产品。PIs已经开始研究枯草杆菌在各种生长条件下的代谢，需要一个可靠的发酵监测系统来扩大他们对细胞对培养环境的生理反应的了解。第二个项目名为“骨髓干细胞的大规模生物反应器生长研究”。该项目将重点开发培养干细胞的程序，并研究干细胞向各种细胞类型的分化。本研究的结果可能对骨髓移植具有重要的实用价值。

This award is to provide funding to develop a fermentation system with the capability to monitor and/or control a number of fermentation parameters. The first project is entitled, " A Study of Bacillus subtilis metabolism." B. subtilis is an important bacterium for hosting cloned foreign genes and for production of a number of extracellular proteins. An important engineering issue is the development of cultivation techniques to achieve high cell density and efficient conversion of substrate to a product. The PIs have begun examining the metabolism of B. subtilis under a wide range of growth conditions and a reliable fermentation monitoring system is required to expand their understanding of cell physiological responses to culture environment. The second project is entitled "Large Scale Bioreactor Growth Studies of Bone Marrow Stem Cells." This project will focus on the development of procedures for growing stem cells and for studying their differentiation to various cell types. The results of this research could have important utility in bone marrow transplantation.