Collaborative Research: Maximizing Therapeutic DNA Process Productivity

合作研究:最大限度地提高治疗性 DNA 工艺效率

基本信息

  • 批准号:
    0967932
  • 负责人:
  • 金额:
    $ 34.84万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
    Standard Grant
  • 财政年份:
    2010
  • 资助国家:
    美国
  • 起止时间:
    2010-05-15 至 2013-04-30
  • 项目状态:
    已结题

项目摘要

0967932AtaaiDNA vaccines are becoming increasingly important as a way to confer immunity. Ultimately, DNA vaccines should be safer and more effective than traditional vaccines, however, currently, there are limitations in our ability to produce the necessary DNA. To meet the demand for DNA vaccines and other DNA products, it would be beneficial to increase the productivity of production processes. While reducing cost, faster processing would also enable DNA vaccine producers to more quickly respond to pathogen mutations and disease outbreaks. The proposed work addresses this problem. The main idea is when host and plasmid DNA are concurrently engineered, DNA production increases dramatically, while the host grows quickly on simple and inexpensive medium. The investigators will use a combination of metabolic engineering, proteomics, NMR, bioprocessing, and mathematical modeling to achieve their goals. The work will extend metabolic engineering practice from the current foci of small molecules and proteins to DNA products, which is important in advancing the field of metabolic engineering. From a more fundamental standpoint, the integration of plasmid DNA synthesis with host cell metabolism, which is not now well understood, will be improved. The effect of combining mutations in the control of plasmid replication is also not yet well explored. The work will reveal the extent to which the effects are synergistic, which would be of interest to structural molecular biologists. The impact of redox cofactor balancing will also be elucidated, which is of current interest to a number of ongoing metabolic engineering efforts. A successful outcome will accelerate the production of therapeutic DNA products and facilitate clinical trials. This collaborative project would provide broad education and training for the graduate and undergraduate students (including minority students) involved in metabolic engineering, computation, NMR, proteomics, and the bioprocessing of DNA products.
AtaaiDNA疫苗作为赋予免疫力的一种方式正变得越来越重要。最终,DNA疫苗应该比传统疫苗更安全,更有效,然而,目前,我们生产必要DNA的能力有限。为了满足对DNA疫苗和其他DNA产品的需求,提高生产过程的生产率将是有益的。在降低成本的同时,更快的处理也将使DNA疫苗生产商能够更快地应对病原体突变和疾病爆发。拟议的工作解决了这个问题。其主要思想是当宿主和质粒DNA同时工程化时,DNA产量急剧增加,而宿主在简单且廉价的培养基上快速生长。研究人员将使用代谢工程,蛋白质组学,NMR,生物加工和数学建模的组合来实现他们的目标。这项工作将把代谢工程实践从目前的小分子和蛋白质的焦点扩展到DNA产品,这对推进代谢工程领域很重要。从更基本的观点来看,质粒DNA合成与宿主细胞代谢的整合将得到改善,这一点现在还没有得到很好的理解。组合突变在控制质粒复制中的作用也尚未得到很好的探索。这项工作将揭示协同效应的程度,这将是结构分子生物学家感兴趣的。氧化还原辅因子平衡的影响也将阐明,这是目前正在进行的代谢工程的努力。成功的结果将加速治疗性DNA产品的生产并促进临床试验。该合作项目将为参与代谢工程、计算、NMR、蛋白质组学和DNA产物生物加工的研究生和本科生(包括少数民族学生)提供广泛的教育和培训。

项目成果

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Mohammad Ataai其他文献

Mohammad Ataai的其他文献

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{{ truncateString('Mohammad Ataai', 18)}}的其他基金

Collaborative Research: Efficient Bioseparation by Intertwining Strain, Chromatography, and Affinity Tail Design
合作研究:通过交织菌株、色谱和亲和尾部设计实现高效生物分离
  • 批准号:
    0533949
  • 财政年份:
    2006
  • 资助金额:
    $ 34.84万
  • 项目类别:
    Continuing Grant
REU Site: Chemical Engineering Research Experience for Undergraduates
REU 网站:本科生化学工程研究经验
  • 批准号:
    0097664
  • 财政年份:
    2001
  • 资助金额:
    $ 34.84万
  • 项目类别:
    Continuing Grant
ME: Metabolic Engineering of Bacillus for Enhanced Product Yield
ME:芽孢杆菌代谢工程以提高产品产量
  • 批准号:
    0118961
  • 财政年份:
    2001
  • 资助金额:
    $ 34.84万
  • 项目类别:
    Continuing Grant
Chemical Engineering Research Experiences for Undergraduates
本科生化学工程研究经历
  • 批准号:
    9732421
  • 财政年份:
    1998
  • 资助金额:
    $ 34.84万
  • 项目类别:
    Continuing Grant
Optimization of IMAC by Fine-Tuning of Protein Metal Interactions
通过微调蛋白质金属相互作用来优化 IMAC
  • 批准号:
    9713286
  • 财政年份:
    1997
  • 资助金额:
    $ 34.84万
  • 项目类别:
    Standard Grant
Mapping and Redirecting Metabolic Fluxes to Attain Significantly Lessen Oxygen Requirements, Acid Production, and Enhanced Recombinant Culture Productivity
映射和重定向代谢通量,以显着减少需氧量、酸产生并增强重组培养生产力
  • 批准号:
    9622821
  • 财政年份:
    1996
  • 资助金额:
    $ 34.84万
  • 项目类别:
    Continuing Grant
Research Experiences for Undergraduates
本科生的研究经历
  • 批准号:
    9322361
  • 财政年份:
    1994
  • 资助金额:
    $ 34.84万
  • 项目类别:
    Continuing Grant
Measuring Metabolic Fluxes: Applications for Elucidating Energetics and Regulation
测量代谢通量:阐明能量学和调节的应用
  • 批准号:
    9207614
  • 财政年份:
    1992
  • 资助金额:
    $ 34.84万
  • 项目类别:
    Continuing Grant
Convinuous Production of Proteins in Bacillus: Secretion byVegetative Cells
芽孢杆菌中蛋白质的连续生产:营养细胞的分泌
  • 批准号:
    9001354
  • 财政年份:
    1990
  • 资助金额:
    $ 34.84万
  • 项目类别:
    Standard Grant
Engineering Research Equipment Grant: On-Line Fermentation Monitoring System
工程研究设备资助:发酵在线监测系统
  • 批准号:
    8907371
  • 财政年份:
    1989
  • 资助金额:
    $ 34.84万
  • 项目类别:
    Standard Grant

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协作研究:CIF:小型:最大化编码计算中的编码增益
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