Convinuous Production of Proteins in Bacillus: Secretion byVegetative Cells

本提案的目的是证明 修饰芽孢杆菌表达系统的可行性， 可以让营养细胞持续生产 分泌蛋白质。 关于这一点，我们知之甚少。 营养细胞合成和分泌的能力 proteins. 修饰的表达系统可以用作 新的重组DNA技术的基础， 生产商业上重要的芽孢杆菌胞外酶， 异源外源蛋白

The objective of this proposal is to demonstrate the feasibility of modifying Bacillus expression systems which will allow vegetative cells to continuously produce and secrete proteins. Very little is known about the capabilities of vegetative cells to synthesize and secrete proteins. The modified expression systems could serve as a basis for new recombinant DNA technology for efficient production of commercially important Bacillus exoenzymes and heterologous foreign proteins.