Acquisition of a Time-Resolved Spectrofluorometer

获取时间分辨分光荧光计

• 批准号: 8907375
• 负责人: Pill-Soon Song
• 金额: $ 5.85万
• 依托单位: University of Nebraska-Lincoln
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1989
• 资助国家: 美国
• 起止时间: 1989-07-01 至 1991-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8907375&HistoricalAwards=false
• 关键词: Acquisition; Time; Resolved; Spectrofluorometer

This proposal requests support to obtain a Time-Resolved Spectrofluorometer. This equipment enables one to measure very fast processes, occurring in the time scales of microsecond to 100 picoseconds, in biological energy conversion and light- induced structural changes in biomolecules and membranes. The proposed instrument will be the only one of its kind at the University of Nebraska. Since this instrument is essential in studying very fast reactions involved in biological energy conversion (as in photosynthesis and photobiology) and structural changes of the biomolecules systems (as biomembranes and proteins), a number of researchers at UNL are expected to make use of this equipment in their research. Graduate students will also be exposed to this equipment for their training and graduate research. The initial experiments anticipated to be performed with this equipment include studies of (1) the phototransformation of phytochrome, a light-regulator of gene expression for the biogenesis of photosynthetic apparatus in green plants, (2) the structure and topography of photosynthetic pigments, and (3) structure-function relationships and dynamic properties of DNA, proteins and model biological membranes. The first two areas of the proposed studies focus on elucidating the primary molecular processes of the tetrapyrrole-based photoreceptors in plant photobiological systems.

本提案要求获得支持， 荧光分光光度计。 这种设备使人们能够测量非常 快速过程，发生在微秒到 100皮秒，在生物能量转换和光- 引起生物分子和膜的结构变化。 拟议的文书将是唯一的一个在这类文书， 内布拉斯加大学。 由于这一工具在 研究生物能源中的快速反应 转换（如光合作用和光生物学）和结构 生物分子系统（如生物膜和 蛋白质），UNL的一些研究人员预计将使 在他们的研究中使用这些设备。 研究生将 也暴露在这些设备中进行培训， 毕业研究。 最初的实验预计将与此进行 设备包括研究（1）光转化的 光敏色素，一种基因表达的光调节剂， 绿色植物光合机构的生物发生;（2）光合作用的生物机制 光合色素的结构和形貌;（3） DNA的结构-功能关系和动力学性质， 蛋白质和模型生物膜。 前两个领域 建议的研究集中在阐明主要分子 植物四吡咯类光感受器的过程 光生物系统