Isolation and Characterization of Neural Crest Subpopulations

神经嵴亚群的分离和表征

• 批准号: 8910987
• 负责人: Margaret Lomax
• 金额: $ 25.08万
• 依托单位: Regents of the University of Michigan - Ann Arbor
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1989
• 资助国家: 美国
• 起止时间: 1989-11-01 至 1993-10-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8910987&HistoricalAwards=false
• 关键词: Isolation; Characterization; Neural; Crest; Subpopulations

The long-range objective of this project is to understand how subpopulations of neural crest cells differentiate into specific types of mature neurons during embryonic development. What genetic and other factors are at work? Dr. Barald and her coworkers have identified subpopulations of cells in the neural crest of chick and quail embryos with different biochemical properties. They have developed markers for these different subpopulations and techniques for separating these cell types while keeping them alive. They can now grow these subpopulations in culture and characterize their responses to a variety of regulatory factors as well as to examine the interaction of various genes important in cellular differentiation. By employing these methods, Dr. Barald's group will be able to answer the following questions: When does a neuronal population that is derived from the neural crest become determined, and therefore segregated from other cells in the crest lineage? Is the expression of the determined state plastic? Can it be modified by environmental cues in vivo or in vitro?

该项目的长期目标是了解神经脊细胞亚群在胚胎发育过程中如何分化为特定类型的成熟神经元。是什么遗传因素和其他因素在起作用？巴拉尔德博士和她的同事们已经在鸡和鹌鹑胚胎的神经脊中确定了具有不同生化特性的细胞亚群。他们已经为这些不同的亚群开发了标记和技术，以便在分离这些细胞类型的同时保持它们的存活。他们现在可以在培养中培养这些亚群，并表征他们对各种调节因子的反应，以及检查在细胞分化中重要的各种基因的相互作用。通过使用这些方法，巴拉尔德博士的团队将能够回答以下问题：从神经脊派生的神经元群体何时变得确定，从而与脊谱系中的其他细胞分离？确定状态的表达是可塑性的吗？它能在体内或体外被环境线索改变吗？