Tissue-Specific Expression of Cytochrome c Oxidase

哺乳动物细胞色素c氧化酶（考克斯）由13个亚基组成： 3个在线粒体DNA中编码，10个在核DNA中编码。 长- 长期目标是批判性地理解这种调节 重要的酶复合物通过分析基因调控 控制考克斯核基因表达的序列。 短期 目标包括定义顺式调节序列元件（cis- 两个考克斯基因的启动子中的元件）：人COX 5 B（对于一个 普遍存在的亚基VB）和牛COX 8H（用于亚基VIII-心脏）。 他们将：（1）通过DNA测序鉴定这些顺式元件， COX 5 B基因的启动子已知对于 其他基因的转录控制;（2）识别独特的顺式- 呼吸基因转录调控的重要元件 通过对COX 5 B启动子的功能分析， DNA酶I超敏位点的检测，B）基因融合实验，和C） 瞬时表达研究：（3）启动子的克隆和序列分析 的牛COX 8H基因，以确定独特的顺式元件的重要 用于组织特异性表达。 这些研究对顺- 控制核表达的调控序列元件 这种独特的酶复合物的基因将有助于我们 了解基因调控信号， 线粒体生物发生和组织中=这种特异性表达 重要的酶复合物。* //

Mammalian cytochrome c oxidase (COX) consists of 13 subunits: three encoded in mitochondrial DNA and 10 in nuclear DNA. Long- term goals are to understand the regulation of this critically important enzyme complex through analysis of the genetic regulatory sequences controlling expression of COX nuclear genes. Short term goals include defining the cis-regulatory sequence elements (cis- elements) in the promoters of two COX genes: human COX5B (for a ubiquitous subunit VB), and bovine COX8H (for subunit VIII-heart). They will: (1) identify by DNA sequencing those cis-elements in the promoter of the COX5B gene known to be important for transcriptional control of other genes; (2) identify unique cis- elements important for transcriptional control of respiratory genes by functional analysis of the COX5B promoter through a) analysis of DNAseI hypersensitive sites, b) gene fusion experiments, and C) transient expression studies; (3) clone and sequence the promoter of the bovine COX8H gene to identify unique cis-elements important for tissue-specific expression. These studies on the cis- regulatory sequence elements controlling expression of nuclear genes for this unique enzyme complex will contribute to our understanding of the genetic regulatory signals involved in mitochondrial biogenesis and in tissue=specific expression of this important enzyme complex.*** //