Regulation of Chloroplast DNA Replication

叶绿体 DNA 复制的调控

• 批准号: 8911485
• 负责人: Madeline Wu
• 金额: $ 27.32万
• 依托单位: University of Maryland Baltimore County
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1989
• 资助国家: 美国
• 起止时间: 1989-09-01 至 1993-02-28
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8911485&HistoricalAwards=false
• 关键词: Regulation; Chloroplast; DNA; Replication

Chloroplasts are autonomously replicating organelles which function to harvest light energy into chemical form in plants. They possess their own DNA and replicating machinery. In this renewal project, Dr. Wu will continue studying the control of DNA replication in the chloroplast of the unicellular green alga, Chlamydomonas. In particular, she will focus on the minimal structural requirements for the DNA replication origin, the development of an in vitro replication system for studying the process, and characterization of a novel 18 kDa iron-sulfur containing membrane protein which exhibits very strong binding to the replication origin and which has electron-transport capability. Some experiments are also proposed using chloroplasts isolated from higher plants, to determine if the information obtained from the algal system are directly relevant to higher plant systems. Chloroplast DNA replicates preferentially in the light phase of the growth cycle. The control of DNA replication initiation, and its relationship to other cellular processes, is not well understood. These proposed experiments promise to shed light on this problem, not only for the green alga, Chlamydomonas, but also for higher plants.

叶绿体是自主复制的细胞器，其功能是将光能转化为植物中的化学形式。 他们拥有自己的DNA和复制机制。 在这个更新项目中，吴博士将继续研究单细胞绿藻衣藻叶绿体中DNA复制的控制。 她将特别关注 DNA 复制起点的最低结构要求、用于​​研究该过程的体外复制系统的开发，以及新型 18 kDa 含铁硫膜蛋白的表征，该膜蛋白与复制起点表现出非常强的结合并具有电子传输能力。 还提出使用从高等植物中分离的叶绿体进行一些实验，以确定从藻类系统获得的信息是否与高等植物系统直接相关。 叶绿体 DNA 优先在生长周期的光照阶段复制。 DNA 复制起始的控制及其与其他细胞过程的关系尚不清楚。 这些拟议的实验有望揭示这个问题，不仅适用于绿藻、衣藻，也适用于高等植物。