Chloroplast and nuclear microsatellite DNA diversities reveal the introduction history of invasive species in Japan

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In order to elucidate the invasion process and effectively manage the growing area of R. pseudoacacia, two studies were carried out as follows :First, to understand the invasion process using nuclear and chloroplast SSR markers. In Japan, there is no isolation by distance, however, significant genetic differences were found between populations. The genetic analysis also suggested that R. pseudoacacia inJapan might be originated from several seed/seedling sources.Second, to reasonably design the growing area size of R. pseudoacacia for honeybee farms, the genotype of pollen collected from honeybee legs was compared with flowered trees growing around honey colonies. From this results, we estimated the foraging area.