Directed Metabolic Flow by Cellular Reduction State Control

拟议工作的目标是研究影响 细胞外还原环境的影响 细胞内还原状态，看看它如何影响 代谢物的流动。 细胞内还原状态 将使用培养物荧光进行测量。 总的来说， 已知NAD（P）H水平调节特定途径 在多产品发酵中， 有证据表明细胞外的氧化还原 电位影响细胞内的NAD（P）H水平。 是 希望利用这种现象来控制细胞， 给定细胞内还原状态， 代谢流优先于其他产品。 的 外部氧化还原电位将由两个 不同的方法，一种是物理的，另一种是化学的。 除了更好地从根本上了解如何 NAD（P）H水平影响终产物的形成， 将有助于发展氧化还原电位和培养荧光 作为在线代谢状态传感器， 用于产品优化的生物反应器操作策略。

The objective of the proposed work is to study the effect of the extracellular reducing environment on the intracellular reduction state and to see how it affects the flow of metabolites. The intracellular reduction state will be measured using culture fluorescence. In general, NAD(P)H levels are known to regulate the particular pathway that is chosen over others in a multi-product fermentation and there is evidence that the extracellular redox potential affects the NAD(P)H level within cells. It is hoped to exploit this phenomenon to control cells at a given intracellular reduction state and thus direct metabolic flow to one product in preference to others. The external redox potential will be controlled by two different methods, one physical and the other chemical. In addition to a better fundamental understanding of how NAD(P)H levels affect end-product formation, this study will help develop redox potential and culture fluorescence as online metabolic state sensors that will lead to novel bioreactor operating strategies for product optimization.