Research Initiation: Neutralization of Oxygen Toxicity in Mammalian Cell Culture Using an Reducing Environment and Free Radical Scavengers

研究启动奖：使用还原环境和自由基清除剂中和哺乳动物细胞培养中的氧毒性摘要拟议的工作将有助于确定哺乳动物细胞培养中的氧毒性问题，并导致细胞培养策略的发展，以减少氧化损伤，从而提高反应堆的生产率。氧化还原电位和荧光作为代谢状态传感器的使用将进一步加深对细胞代谢的了解，并有助于开发重要的非侵入性在线分析监测方法。此外，通过合作努力，杂交瘤中由繁殖和自由基损伤引起的体细胞突变将被检测。

RESEARCH INITIATION AWARD: NEUTRALIZATION OF OXYGEN TOXICITY IN MAMMALIAN CELL CULTURE USING A REDUCING ENVIRONMENT AND FREE RADICAL SCAVENGERS ABSTRACT The proposed work will serve to define problems of oxygen toxicity in mammalian cell culture and lead to the development of cell culturing strategies to decrease oxidative damage and thereby increase reactor productivities. The use of redox potential and fluorescence as metabolic state sensors will further the understanding of cellular metabolism and assist in the development of the important non-invasive, online analytical monitoring methods. Additionally, through collaborative efforts, somatic mutagenesis in hybridomas caused by propagation as well as by free radical damage will be examined.