US-Sweden Cooperative Research: Mathematical Studies of Dopamine and Microdialysis

该奖项将支持约瑟夫博士之间的合作研究 司法部，埃默里大学和Urban Ungerstadt博士， 药理学，Karolinska研究所，斯德哥尔摩，瑞典。 的 本研究的目的是探讨多巴胺神经化学 为了更好地表征微透析，一种新的监测方法 多巴胺和其他神经递质的化学成分，代谢物 和相关化合物。 在这个项目中合作的两个实验室是领先的 那些参与了快速发展的体内技术， 透析 这项技术已经被证明是有用的， 检查细胞外液的化学成分， 个脑袋 拟议的研究将继续调查 透析对周围组织的影响，研究方法 将获得的化学信息与突触 区域，并特别检查儿茶酚摄取的作用- 多巴胺能神经元中的地雷。 该项目将涉及- 多巴胺能神经末梢的理论建模。 的新数据 这两个实验室将被纳入该模型。 摄取 并且抑制摄取将是工作的特别重点。 在 此外，微透析取样方法在这两个使用 研究多巴胺的实验室将被表征。 字符- 化将包括扩散的数学分析， 在细胞外液采样期间创建的配置文件。 这 将提供有关一项技术的重要新信息， 在美国的许多实验室里， 和欧洲

This award will support collaborative research between Dr. Joseph Justice, Emory University, and Dr. Urban Ungerstadt, Department of Pharmacology, Karolinska Institute, Stockholm, Sweden. The objective of the research is to investigate dopamine neurochemistry and to better characterize microdialysis, a new method of monitoring the chemistry of dopamine and other neuro-transmitters, metabolites and related compounds. The two laboratories cooperating in this project are the leading ones involved in the rapidly emerging technique of in vivo dialysis. This technique has already been shown to be useful to examine the chemical composition of the extracellular fluid of the brain. The proposed research will continue the investigation of the effect of dialysis on the surrounding tissue, investigate ways to relate the chemical information obtained to the synaptic region, and specifically examine the role of uptake of catechola- mines in dopaminergic neurons. The project will involve mathema- tical modeling of the dopaminergic nerve terminal. New data from the two laboratories will be incorporated into the model. Uptake and inhibition of uptake will be a particular focus of effort. In addition, the microdialysis sampling methodology in use in both laboratories to study dopamine will be characterized. The charac- terization will consist of a mathematical analysis of the diffusion profiles created during sampling of the extracellular fluid. This will provide important new information about a technique that is being increasingly used in many laboratories in the United States and Europe.