Micellar Mobile Phases in Liquid Chromatography

该项目属于分析化学和表面化学的一般领域，以及液相色谱子领域。本实验计划的重点是对反相液相色谱(RPLC)中的阴离子、阳离子和两性离子胶束流动相以及正相液相色谱(NPLC)中的反(反)胶束流动相进行广泛、系统的研究。这项活动是使用胶束流动相对RPLC选择性的首次全面研究，NPLC的研究特别值得注意，因为它们可以通过控制系统中的水分含量来导致NPLC的更广泛使用，并通过这种强大的色谱方法可靠地使用安培检测。除了有助于加强对基础色谱科学的理解外，这项研究还可能带来对生物技术研究和制造极其重要的新的、通用的分析技术。

This project is in the general area of analytical and surface chemistry and in the subfield of liquid chromatography. The thrust of this experimental program focusses on broad, systematic studies of anionic, cationic, and zwitterionic micellar mobile phases in reverse phase liquid chromatography (RPLC), and of reversed (inverted) micellar mobile phases in normal phase liquid chromatography (NPLC). This activity is the first comprehensive study of RPLC selectivity using micelle mobile phases and the NPLC studies are particularly note worthy in that they could lead to the wider use of NPLC through control of water content in the system and to the reliable use of amperometric detection with this powerful chromatographic method. In addition to contributing to an enhanced understanding of fundamental chromatographic science, this research could lead to new and versatile analytical techniques of immense importance to biotechnology research and manufacturing.