Studies of the Primary Reactions of Bacterial Photosynthesis

细菌光合作用初级反应的研究

• 批准号: 8917729
• 负责人: Neal Woodbury
• 金额: $ 29.85万
• 依托单位: Arizona State University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1990
• 资助国家: 美国
• 起止时间: 1990-04-01 至 1993-09-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8917729&HistoricalAwards=false
• 关键词: Studies; Primary; Reactions; Bacterial; Photosynthesis

The primary goal of this research is to gain insight into the role that the L and M polypeptides of the bacterial photosynthetic reaction center play in determining the parameters of electron transfer between reaction center chromophores. To this end, sophisticated spectroscopic techniques will be combined with molecular genetic manipulation of the amino acid sequences of these polypeptides for the analysis of reaction center function. Specific methods to be employed include picosecond resolution fluorescence decay measurements, subpicosecond resolution transient absorption spectroscopy of electron transfer intermediates, cassette mutagenesis of reaction center genes, and the production of chimeric reaction centers by forcing deletions in the reaction center genes of one organism to be repaired by reaction center sequences derived from a related organism. This work will result in a better understanding of the specific electron transfer reactions involved in the primary events of photosynthesis and provide new insights into the dielectric nature of proteins in general.

这项研究的主要目标是深入了解 细菌的L和M多肽 光合反应中心在决定 反应中心间电子转移参数 发色团 为此， 技术将与分子遗传学相结合， 这些蛋白质的氨基酸序列的操作 用于分析反应中心功能的多肽。 具体采用的方法包括皮秒分辨率 荧光衰减测量，亚皮秒分辨率 电子转移瞬态吸收光谱 中间体，反应中心基因的盒式诱变， 和嵌合反应中心的产生， 一个生物体的反应中心基因缺失， 由来自相关的反应中心序列修复 有机体 这项工作将使人们更好地了解 参与的特定电子转移反应 光合作用的主要事件，并提供新的见解 蛋白质的介电性质。