Prevalence of Immune Response for Ebola in the Endemic Populations

流行人群中埃博拉免疫反应的发生率

• 批准号: 1518528
• 负责人: Neal Woodbury
• 金额: $ 20万
• 依托单位: Arizona State University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-12-15 至 2015-11-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1518528&HistoricalAwards=false
• 关键词: Prevalence; Immune; Response; Ebola; Endemic

This NSF Rapid response Research (RAPID) project will examine the differences in immune response between individuals who live or die after infection with the Ebola virus. Currently it is not well understood why some infected individuals live while others die. It is important to understand how the response of immune system develops before the symptoms of the disease appears. In this proposal, investigators will examine blood samples from Ebola patients to look for proteins associated with immune response, and examine the differences in these immune proteins between patients who live versus patients who die after infection. They will also examine the time course of the appearance of immune proteins. Early detection of changes in these immune proteins will allow for the identification of the individuals, such as health care workers, already exposed to the virus. Such screening of the immune system may also lead to discovery of antibodies that are produced by the immune system of some individuals who survived the disease. These antibodies can be used for vaccine development or be used directly in infected individuals as a therapeutic.Technical: The goal of this NSF Rapid response Research (RAPID) project is to determine the immunosignature of individuals who have survived the Ebola infection and to examine the time course of the immunosignature during infection prior to the development of symptoms. The PI will use a technology developed in his laboratory that employs high density arrays of peptides to detect antibody binding using a less than a drop of blood. The pattern of binding depends on the immunological state or health status of the individual. The PI has collaborators in place with access to blood samples from previous Ebola outbreaks, as well as access to samples from the current Ebola outbreak. This work should lead to the development of methodologies for early detection of Ebola infection prior to the development symptoms, and could advance vaccine and therapeutic development.

NSF 快速反应研究 (RAPID) 项目将研究感染埃博拉病毒后存活或死亡的个体之间免疫反应的差异。目前尚不清楚为什么一些感染者存活而另一些人死亡。了解在疾病症状出现之前免疫系统的反应如何发展非常重要。在这项提案中，研究人员将检查埃博拉患者的血液样本，寻找与免疫反应相关的蛋白质，并检查感染后存活的患者与死亡的患者之间这些免疫蛋白的差异。 他们还将检查免疫蛋白出现的时间过程。 及早检测这些免疫蛋白的变化将有助于识别已经接触过该病毒的个体，例如医护人员。这种对免疫系统的筛查还可能导致发现一些在疾病中幸存的个体的免疫系统产生的抗体。这些抗体可用于疫苗开发或直接用于感染个体作为治疗剂。 技术：NSF 快速反应研究 (RAPID) 项目的目标是确定埃博拉感染幸存者的免疫特征，并检查感染期间出现症状之前免疫特征的时间过程。 PI 将使用其实验室开发的一项技术，该技术采用高密度肽阵列，只需不到一滴血即可检测抗体结合。结合模式取决于个体的免疫状态或健康状况。 PI 拥有适当的合作者，可以获取之前埃博拉疫情爆发的血液样本，以及当前埃博拉疫情爆发的样本。 这项工作应该有助于开发出在出现症状之前早期检测埃博拉感染的方法，并可以推进疫苗和治疗方法的开发。