Heat and Mass Transfer Processes During Freezing of Biological Tissue

生物组织冷冻过程中的传热传质过程

• 批准号: 8918863
• 负责人: Boris Rubinsky
• 金额: $ 17.5万
• 依托单位: University of California-Berkeley
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1990
• 资助国家: 美国
• 起止时间: 1990-05-01 至 1993-04-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8918863&HistoricalAwards=false
• 关键词: Heat; Mass; Transfer; Processes; During

This program deals with heat and mass transfer phenomena in the freezing of mammalian biological tissue and to provide a fundamental understanding of the thermophysical processes that occur during freezing of tissue. This study will be important to the development of methods for long-term storage of frozen organs, organs to be used for transplantation of other purposes. The method to be employed involves the correlation of the structure of the mammalian tissue after freezing to the perfusion treatments prior to freezing and the thermal history during freezing. Freezing and thawing will be performed under a range of controlled thermal conditions with a special "directional solidification: apparatus developed by the authors. The structure and chemical composition of samples will be assessed with Low-Temperature, Scanning Electron Microscopy, High Voltage Electron Microscopy, and X-Ray Microanalysis. As an outgrowth of a recent expedited award, the PI's will perfuse biological organs, specifically heart, kidney, and liver, with antifreeze glycopeptides, and study the role of this promising substance on the processes of tissue freezing. The experimental results will be used to develop mathematical models for the process of the freezing of tissue.

该程序处理中的传热和传质现象 哺乳动物生物组织的冷冻，并提供一种 对热物理过程的基本理解， 发生在组织冷冻过程中。 这项研究将是重要的 长期保存冷冻食品的方法 器官，器官用于其他器官移植 目的 所采用的方法涉及的相关性， 冷冻后的哺乳动物组织的结构 冷冻前的灌注处理和热历史 在冻结期间。 冷冻和解冻将在 一系列受控的热条件， “定向凝固： 自行 样品的结构和化学成分 将使用低温扫描电子显微镜进行评估 显微镜、高压电子显微镜和X射线 微量分析。 作为最近一项快速裁决的结果， PI会灌注生物器官，特别是心脏 肾脏和肝脏，与抗冻糖肽， 这种有前途的物质在以下过程中的作用 组织冷冻 实验结果将用于 建立冷冻过程的数学模型， 组织.