Heat and Mass Transfer Processes During Freezing of Biological Tissue

本课程研究哺乳动物生物组织在冷冻过程中的热和质传递现象，并提供对组织冷冻过程中发生的热物理过程的基本了解。这项研究将对长期保存冷冻器官的方法的发展具有重要意义，用于其他目的移植的器官。所采用的方法包括冷冻后哺乳动物组织的结构与冷冻前的灌流处理以及冷冻期间的热历史的相关性。冻结和解冻将在一系列受控的热条件下进行，并使用作者开发的特殊“定向凝固：装置”。样品的结构和化学成分将通过低温、扫描电子显微镜、高压电子显微镜和X射线显微分析进行评估。作为最近一项加速奖的结果，PI将向生物器官，特别是心脏、肾脏和肝脏灌流抗冻剂糖肽，并研究这种有希望的物质在组织冻结过程中的作用。实验结果将被用来建立组织冻结过程的数学模型。

This program deals with heat and mass transfer phenomena in the freezing of mammalian biological tissue and to provide a fundamental understanding of the thermophysical processes that occur during freezing of tissue. This study will be important to the development of methods for long-term storage of frozen organs, organs to be used for transplantation of other purposes. The method to be employed involves the correlation of the structure of the mammalian tissue after freezing to the perfusion treatments prior to freezing and the thermal history during freezing. Freezing and thawing will be performed under a range of controlled thermal conditions with a special "directional solidification: apparatus developed by the authors. The structure and chemical composition of samples will be assessed with Low-Temperature, Scanning Electron Microscopy, High Voltage Electron Microscopy, and X-Ray Microanalysis. As an outgrowth of a recent expedited award, the PI's will perfuse biological organs, specifically heart, kidney, and liver, with antifreeze glycopeptides, and study the role of this promising substance on the processes of tissue freezing. The experimental results will be used to develop mathematical models for the process of the freezing of tissue.