The Na+ Cycle of Extremely Alkalophilic Bacilli

极嗜碱杆菌的钠循环

• 批准号: 8919510
• 负责人: Terry Krulwich
• 金额: --
• 依托单位: CUNY Mount Sinai School of Medicine
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1990
• 资助国家: 美国
• 起止时间: 1990-02-01 至 1991-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8919510&HistoricalAwards=false
• 关键词: Na+; Cycle; Extremely; Alkalophilic; Bacilli

The sodium ion cycle of extremely alkalophilic bacilli is a simple cycle of secondary porters: an electrogenic sodium ion/proton antiporter that catalyzes accumulation of protons (pH homeostasis) and extrusion of sodium ion, and functions secondary to the proton-pumping respiratory chain; and sodium ion/solute symporters that couple solute accumulation to sodium ion re-entry down its electrochemical gradient. The physiology of the cycle is well-characterized, but the molecular properties of the porters and their regulation are unexplored. This project develops genetic approaches that facilitates a molecular characterization. In addition, the gene encoding the antiporter will be cloned. This action funds a visit of an Israeli scientist to collaborate on the cloning of a gene for a transporter protein in bacteria capable of living at extreme pH levels. This transporter plays an integral role in regulating internal acid levels and controls the organism's ability to adapt to extreme environments.

极端嗜碱杆菌的钠离子循环是一个简单的次级转运体循环：电致钠离子/质子反转运体，催化质子的积累（pH稳态）和钠离子的挤压，作用于质子泵吸呼吸链；钠离子/溶质同调体将溶质积累与钠离子重新进入电化学梯度结合起来。这个周期的生理特性已经被很好地描述了，但是搬运工的分子特性及其调控尚未被探索。本项目开发有利于分子表征的遗传方法。此外，编码反转运蛋白的基因将被克隆。这一行动资助了一位以色列科学家的访问，以合作克隆能够在极端pH水平下生存的细菌中的一种转运蛋白基因。这种转运体在调节体内酸水平和控制生物体适应极端环境的能力方面起着不可或缺的作用。