Improvement in Immunology and Biotechnology Laboratory Instruction

该奖项为阿拉巴马大学微生物学系提供资金，用于继续和改进两门相关课程的实验室教学：免疫学实验室和细胞培养与杂交瘤技术。这两门课程都是基于项目的密集实验室体验，旨在演示许多现代实验室程序的基本原理。不可靠或无法获得适当的设备，危及已建立的免疫学实验室的继续和改进，以及在新设立的杂交瘤课程的初步试验提供之后继续下去。该奖项将购买的设备包括：两个细胞培养工作站(层流生物安全柜、倒置相差显微镜、水浴)和一个用于细胞培养和杂交瘤技术的双室水套二氧化碳孵化器；四个紫外可见光谱仪、聚丙烯酰胺凝胶电泳设备和一个低速大容量冷藏离心机，用于免疫实验室。这两门课程的结合将向学生介绍现代免疫学技术，然后将这些技术应用于生产和使用单抗的专业领域。虽然完成这次培训的学员数量相对较少，但培训的强度很高。此外，这些设备将通过引入新的演示和扩展实验来影响更多低级课程的学生，并将加强本科生的研究计划。受赠人正在将该奖项与来自非联邦来源的资金相匹配。

This award provides funds to the Department of Microbiology at the University of Alabama for the continuation and improvement of laboratory instruction in two related courses: Immunology Laboratory, and Cell Culture and Hybridoma Technology. Both courses are intensive project-based laboratory experiences designed to demonstrate the underlying principles of many modern laboratory procedures. The unreliability or unavailability of appropriate equipment endangers the continuation and improvement of the well- established Immunology Laboratory and the continuation beyond its initial trial offering of the newly created hybridoma course. Equipment to be purchased with this award includes: two cell culture work stations (laminar flow biological safety cabinet, inverted phase contrast microscope, waterbath) and a double- chambered water-jacketed CO2 incubator for Cell Culture and Hybridoma Technology; and four UV/visible spectrophotometers, polyacrylamide gel electrophoresis equipment, and a low-speed high- volume refrigerated centrifuge for Immunology Laboratory. The combination of these two courses will provide students with an introduction to modern immunologic techniques followed by the applications of these techniques to the specialized field of production and use of monoclonal antibodies. While the number of students that will complete this training is relatively low, the intensity of training is very high. In addition, the equipment will impact upon a larger number of students in lower division courses through the introduction of new demonstrations and expanded experiments and will enhance the undergraduate research program. The grantee is matching this award with funds from non-Federal sources.