Improvement in Immunology and Biotechnology Laboratory Instruction

该奖项为阿拉巴马大学微生物系提供资金，用于继续和改进两门相关课程的实验室教学：免疫学实验室和细胞培养和杂交瘤技术。这两门课程都是密集的基于项目的实验室经验，旨在展示许多现代实验室程序的基本原则。适当设备的不可靠或不可用危及建立良好的免疫学实验室的继续和改进，以及其新创建的杂交瘤课程的最初试验之外的继续。该奖项将购买的设备包括：两个细胞培养工作站（层流生物安全柜、倒置相差显微镜、水浴）和一个用于细胞培养和杂交瘤技术的双室水套CO2培养箱；紫外/可见分光光度计4台，聚丙烯酰胺凝胶电泳设备1台，免疫学实验室低速大容量冷冻离心机1台。这两门课程的结合将为学生提供现代免疫学技术的介绍，然后将这些技术应用于单克隆抗体的生产和使用的专业领域。虽然完成培训的学生数量相对较少，但培训的强度非常高。此外，通过引入新的演示和扩展的实验，该设备将影响更多的低级别课程的学生，并将加强本科研究计划。受赠人将与非联邦来源的资金相匹配。

This award provides funds to the Department of Microbiology at the University of Alabama for the continuation and improvement of laboratory instruction in two related courses: Immunology Laboratory, and Cell Culture and Hybridoma Technology. Both courses are intensive project-based laboratory experiences designed to demonstrate the underlying principles of many modern laboratory procedures. The unreliability or unavailability of appropriate equipment endangers the continuation and improvement of the well- established Immunology Laboratory and the continuation beyond its initial trial offering of the newly created hybridoma course. Equipment to be purchased with this award includes: two cell culture work stations (laminar flow biological safety cabinet, inverted phase contrast microscope, waterbath) and a double- chambered water-jacketed CO2 incubator for Cell Culture and Hybridoma Technology; and four UV/visible spectrophotometers, polyacrylamide gel electrophoresis equipment, and a low-speed high- volume refrigerated centrifuge for Immunology Laboratory. The combination of these two courses will provide students with an introduction to modern immunologic techniques followed by the applications of these techniques to the specialized field of production and use of monoclonal antibodies. While the number of students that will complete this training is relatively low, the intensity of training is very high. In addition, the equipment will impact upon a larger number of students in lower division courses through the introduction of new demonstrations and expanded experiments and will enhance the undergraduate research program. The grantee is matching this award with funds from non-Federal sources.