Modern Immunodetection: ELISA and Electroblotting

现代免疫检测：ELISA 和电印迹

• 批准号: 8951633
• 负责人: Karin Grimnes
• 金额: --
• 依托单位: Alma College
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1989
• 资助国家: 美国
• 起止时间: 1989-03-01 至 1991-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8951633&HistoricalAwards=false
• 关键词: Modern; Immunodetection; ELISA; Electroblotting

The immunology laboratory experience will be improved by utilizing a process-oriented approach. This will be accomplished by introducing equipment for modern protein microanalysis, specifically for the analysis of antibodies and antigens. This equipment includes an enzyme-linked immunosorbant assay (ELISA) reader coupled to a Macintosh computer, and polyacrylamide gel electrophoresis units (mini-PAGE) with drop-in electroblotting cassettes. This equipment will be used to duplicate several important immunological discoveries, and then be used for independent work. Experiments will include quantification of the immune response, characterization of the physical properties of antibodies, and determination of cell cooperation in the immune response. The equipment will also be available for student research projects and will introduced to students in the freshman biology course. The college will provide 50% matching funds.

利用过程导向的方法将提高免疫学实验室的经验。这将通过引入现代蛋白质微量分析设备，特别是抗体和抗原的分析来实现。该设备包括连接到Macintosh计算机的酶联免疫吸附测定（ELISA）阅读器，以及带有插入式电印迹盒的聚丙烯酰胺凝胶电泳装置（mini-PAGE）。该设备将用于重复几个重要的免疫学发现，然后用于独立工作。实验将包括免疫反应的量化，抗体物理特性的表征，以及免疫反应中细胞合作的确定。该设备还将用于学生的研究项目，并将在新生生物课程中介绍给学生。学院将提供50%的配套资金。