Modern Immunodetection: ELISA and Electroblotting

免疫学实验室的经验将通过采用面向过程的方法得到改善。这将通过引进现代蛋白质微量分析设备来实现，特别是抗体和抗原的分析。该设备包括一个与Macintosh计算机相连的酶联免疫吸附分析(ELISA)读取器，以及带有插入式电印盒的聚丙烯酰胺凝胶电泳盒(mini-PAGE)。该设备将用于复制几项重要的免疫学发现，然后用于独立工作。实验将包括免疫反应的量化，抗体物理性质的表征，以及免疫反应中细胞合作的确定。这些设备还将用于学生的研究项目，并将在新生生物课程中介绍给学生。学院将提供50%的配套资金。

The immunology laboratory experience will be improved by utilizing a process-oriented approach. This will be accomplished by introducing equipment for modern protein microanalysis, specifically for the analysis of antibodies and antigens. This equipment includes an enzyme-linked immunosorbant assay (ELISA) reader coupled to a Macintosh computer, and polyacrylamide gel electrophoresis units (mini-PAGE) with drop-in electroblotting cassettes. This equipment will be used to duplicate several important immunological discoveries, and then be used for independent work. Experiments will include quantification of the immune response, characterization of the physical properties of antibodies, and determination of cell cooperation in the immune response. The equipment will also be available for student research projects and will introduced to students in the freshman biology course. The college will provide 50% matching funds.