Acquisition of a Kinetic Flash Spectrophotometer/FluorimeterLuminometer

需要资金建造一台高灵敏度、高时间分辨率的闪光分光光度计，能够测量紫外光、可见光和近红外波长。使用闪光灯来询问吸收信号，从而获得极高的光学分辨率和纳秒时间分辨率。使用光纤导轨将光引导到实验样品中和从实验样品中引出，使得仪器可以很容易地重新配置，以测量荧光和发光，所有这些都使用基本上相同的敏感电子元件。这种类型的仪器经常用于光合作用物质的生物物理研究。因此，四个主要用户和一个次要用户将集中在光合作用参数上，以评估蛋白质跨生物膜运输的模型、光合作用物质对变化的光条件的响应、光合作用电子传输链的组织以及叶绿体和细胞表面之间的细胞内通信。与光合作用没有直接关系的仪器的建议用途包括测定重组膜蛋白周围模型膜的粘度，研究光敏色素脱辅基蛋白突变对光敏色素功能的影响，以及开发灵敏的电生ATPase功能测试方法。

Funds are requested to construct a high sensitivity, high time resolution flash spectrophotometer capable of measurements at UV, visible and near IR wavelengths. A flash lamp is used for interrogation of absorption signals, resulting in extremely high optical resolution coupled with nanosecond time resolution. The use of fiber optic guides to direct light into and away from experimental samples allows the instrument to be easily reconfigured to measure fluorescence and luminescence, all of which make use of essentially the same sensitive electronic components. This type of instrument is used often for biophysical studies of photosynthetic material. Accordingly, four of the major and one of the minor users will concentrate on photosynthetic parameters to evaluate models of protein translocation across biological membranes, responses of photosynthetic material to changing light conditions, organization of the photosynthetic electron transport chain, and intracellular communication between chloroplasts and cell surfaces. Proposed uses for instrument not directly related to photosynthesis include determination of the viscosity of model membranes around reconstituted membrane proteins, investigation of the effects of mutations in the phytochrome apoprotein on phytochrome function, and development of sensitive assays for the function of electrogenic ATPases.