Acquisition of a Kinetic Flash Spectrophotometer/FluorimeterLuminometer

购买动态闪光分光光度计/荧光计发光计

• 批准号: 9002040
• 负责人: Steven Theg
• 金额: $ 4.32万
• 依托单位: University of California-Davis
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1990
• 资助国家: 美国
• 起止时间: 1990-07-01 至 1993-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9002040&HistoricalAwards=false
• 关键词: Acquisition; Kinetic; Flash; Spectrophotometer; FluorimeterLuminometer

Funds are requested to construct a high sensitivity, high time resolution flash spectrophotometer capable of measurements at UV, visible and near IR wavelengths. A flash lamp is used for interrogation of absorption signals, resulting in extremely high optical resolution coupled with nanosecond time resolution. The use of fiber optic guides to direct light into and away from experimental samples allows the instrument to be easily reconfigured to measure fluorescence and luminescence, all of which make use of essentially the same sensitive electronic components. This type of instrument is used often for biophysical studies of photosynthetic material. Accordingly, four of the major and one of the minor users will concentrate on photosynthetic parameters to evaluate models of protein translocation across biological membranes, responses of photosynthetic material to changing light conditions, organization of the photosynthetic electron transport chain, and intracellular communication between chloroplasts and cell surfaces. Proposed uses for instrument not directly related to photosynthesis include determination of the viscosity of model membranes around reconstituted membrane proteins, investigation of the effects of mutations in the phytochrome apoprotein on phytochrome function, and development of sensitive assays for the function of electrogenic ATPases.

要求资金建设高灵敏度、高时效 分辨率闪光分光光度计，能够在UV下测量， 可见光和近红外波长。 闪光灯用于 询问吸收信号，导致极高的 光学分辨率加上纳秒时间分辨率。 的 使用光导纤维引导光进入和离开 实验样品使仪器可以很容易地 重新配置以测量荧光和发光，所有这些 利用基本上相同的敏感电子元件。 这种类型的仪器通常用于生物物理研究， 光合物质 因此，四个主要和一个 的小用户将集中在光合参数 为了评估跨生物学的蛋白质易位模型， 膜，光合物质对变化的光的反应 光合作用电子传递的条件、组织 链和叶绿体之间的细胞内通讯， 细胞表面 不直接相关的文书的拟议用途 光合作用包括模型粘度的测定 重组膜蛋白周围的膜， 光敏色素脱辅基蛋白突变对 光敏色素的功能，并开发敏感的测定方法， 产电ATP酶的功能。