Probing the Interaction of Precursors with the Chloroplast Import Machinery

探讨前体与叶绿体输入机械的相互作用

• 批准号: 1330321
• 负责人: Steven Theg
• 金额: $ 30万
• 依托单位: University of California-Davis
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-11-15 至 2016-10-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1330321&HistoricalAwards=false
• 关键词: Probing; Interaction; Precursors; Chloroplast; Import

While precursors proteins were thought to be transported into plant chloroplasts in an unfolded conformation, recent discoveries indicate that this question is far from settled. The mechanism of protein import into chloroplasts will be probed and the conformation of precursor proteins will be monitored as they are being imported. Fluorescent probes that are sensitive to their local environment will be used to monitor the conformation of substrate proteins as they are captured in the chloroplast import machinery and to determine the size limits of the channels through which import occurs. The upper size limit of the chloroplast import mechanism will be probed using gold particles of fixed sizes.Broader impacts: A program entitled "Fellowships in Molecular Biology at UC-Davis" will bring two college-bound students from the River City High School in West Sacramento to participate in this project for four weeks during each of the two summers of the funding period. Students will be nominated by their AP biology teachers and will work on projects that can be completed in four weeks. Local high school students will be hosted for their Biotechnology class internship during the spring quarter, and a Young Scholars Program student will have the opportunity to work on this project during the summers.

虽然前体蛋白被认为是以未折叠的构象被运输到植物叶绿体中，但最近的发现表明，这个问题远未解决。蛋白质输入叶绿体的机制将被探讨，前体蛋白质的构象将被监测，因为他们正在输入。对它们的局部环境敏感的荧光探针将用于监测底物蛋白的构象，因为它们在叶绿体进口机器中被捕获，并确定进口发生的通道的大小限制。更广泛的影响：一个名为“加州大学戴维斯分校分子生物学奖学金”的项目将在资助期的两个夏天里，让来自西萨克拉门托河城高中的两名大学生参加这个项目，为期四周。学生将由他们的AP生物老师提名，并将在四周内完成的项目工作。当地的高中生将在春季学期进行生物技术类实习，青年学者计划的学生将有机会在夏季从事这个项目。