New Genetic system for the study of the plasid genome

用于研究质粒基因组的新遗传系统

• 批准号: 9004054
• 负责人: Pal Maliga
• 金额: $ 35.56万
• 依托单位: Rutgers University New Brunswick
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1990
• 资助国家: 美国
• 起止时间: 1990-08-01 至 1994-01-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9004054&HistoricalAwards=false
• 关键词: New; Genetic; system; study; plasid

We shall develop the tools for introducing genes into the plastid genome of higher plants using Nicotiana tabacum, a suitable model species. Lack of plastid transformation in higher plants has been due to problems of a large plastid genome copy number in a cell, lack of appropriate selective markers, and an effective way of DNA delivery. First, we shall transform wild-type plastids with plastid 16S rRNA genes that confer resistance to antibiotics. Introduction of DNA will be by bombardment with DNA-coated tungsten particles. Transformation will result in gene replacement. Research will be extended to study the requirements of inserting foreign DNA into the plastid genome. Oligonucleotide, and gene- size segments of DNA will be linked to the selective markers. Incorporation of oligonucleotides will be used to select suitable sites for gene insertion. Larger segments of DNA will include chimeric genes. Involvement of copy correction will be determined in the formation of stable transgenic plastid genomes. Information derived from proposed research will make feasible the study of plastid gene regulation and function in transgenic plants. This research will develop a plastid transformation protocol for higher plants. This is a very important tool for further genetic engineering of plants.

我们将开发将基因引入质体的工具 高等植物的基因组使用烟草，一个合适的模型 物种 高等植物缺乏质体转化， 由于细胞中质体基因组拷贝数大的问题， 缺乏合适的选择标记，缺乏有效的DNA 交付. 首先，我们将野生型质体转化为 质体16 S rRNA基因赋予抗生素抗性。 DNA的引入将通过DNA包被钨的轰击来实现 粒子 转化将导致基因替换。 研究将扩展到研究插入的要求 外源DNA进入质体基因组。 寡核苷酸和基因- 大小的DNA片段将与选择性标记相关联。 寡核苷酸的掺入将用于选择合适的寡核苷酸。 基因插入位点。 较大的DNA片段将包括 嵌合基因 是否涉及复印件更正， 在稳定的转基因质体基因组的形成。 信息 从拟议的研究中得出的结论将使研究可行， 转基因植物中质体基因的调控和功能。 这项研究将开发一种质体转化方案， 高等植物 这是一个非常重要的工具， 植物工程