A Genetic Approach to Study Nuclear-Plastid Interactions in Arabidopsis

9305037 Maliga：拟议的研究是关于核质相互作用的一般领域：目的是识别控制拟南芥单个叶绿体基因表达的核基因。本研究的具体目标是：(1)克隆拟南芥叶绿体rbcL区和PSBA区并测序。(Ii)在PSBA调控序列的控制下，检测编码B-葡萄糖醛酸酶和荧光素酶的报告基因的表达。(3)拟南芥种子诱变和报告基因(uidA、Luc)表达改变的M2代核突变体的筛选。(4)建立互补群，确定突变图谱位置。*通过该项目，将开发操作拟南芥叶绿体基因组的方法，以便叶绿体分子生物学和遗传学也可以从这个模型系统中受益。%%%

9305037 Maliga The proposed research is on the general area of nucleocytoplasmic interactions: The objective is to identify nuclear genes that control the expression of single Arabidopsis thaliana plastid genes. Specific objectives of the research are: (i) Clone and sequence the plastid rbcL and psbA regions from Arabidopsis. (ii) Test expression of reporter genes encoding B- glucuronidase and luciferase under control of psbA regulatory sequences. (iii) Arabidopsis seed mutagenesis and screening of M2 seedlings for nuclear mutants with altered reporter gene (uidA, luc) expression. (iv) Established complementation groups, determine map position of mutations. *** Through the project, methods will be developed for the manipulation of the Arabidopsis plastid genome so that plastid molecular biology and genetics can also benefit from this model system. %%%