A Genetic Approach to Study Nuclear-Plastid Interactions in Arabidopsis

拟开展的研究是关于核胞质相互作用的一般领域：目的是鉴定控制拟南芥质体基因表达的核基因。本研究的具体目标是：(1)拟南芥质体rbcL和psbA区域的克隆和测序。（ii）检测编码B-葡萄糖醛酸酶和荧光素酶的报告基因在psbA调控序列控制下的表达情况。（iii）拟南芥种子诱变及M2幼苗中报告基因（uidA, luc）表达改变的核突变体筛选。（iv）建立互补组，确定突变的图谱位置。***通过该项目，将开发拟南芥质体基因组的操作方法，使质体分子生物学和遗传学也能从该模型系统中受益。% % %

9305037 Maliga The proposed research is on the general area of nucleocytoplasmic interactions: The objective is to identify nuclear genes that control the expression of single Arabidopsis thaliana plastid genes. Specific objectives of the research are: (i) Clone and sequence the plastid rbcL and psbA regions from Arabidopsis. (ii) Test expression of reporter genes encoding B- glucuronidase and luciferase under control of psbA regulatory sequences. (iii) Arabidopsis seed mutagenesis and screening of M2 seedlings for nuclear mutants with altered reporter gene (uidA, luc) expression. (iv) Established complementation groups, determine map position of mutations. *** Through the project, methods will be developed for the manipulation of the Arabidopsis plastid genome so that plastid molecular biology and genetics can also benefit from this model system. %%%