U.S.-Italy Cooperative Research: The Role of Peptide Fragments of Proteins in Folding and Assembly

美意合作研究：蛋白质肽片段在折叠和组装中的作用

• 批准号: 9004111
• 负责人: Harold Scheraga
• 金额: $ 1.65万
• 依托单位: Cornell University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1990
• 资助国家: 美国
• 起止时间: 1990-08-15 至 1994-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9004111&HistoricalAwards=false
• 关键词: U.S.; Italy; Cooperative; Research; Role

This award will support Professor Harold Scheraga of Cornell University and Professor George Nemethy of the Mount Sinai School of Medicine in a research collaboration with Professor Adriana Zagari of the University of Naples, Italy. The purpose of the collaboration is the determination of the preferred conformations of several short peptides that form structurally and functionally important parts of proteins. Two specific problems will be investigated. The first problem is that of the nonhelical telopeptides of collagen. These peptides play an important role in directing and stabilizing the particular manner of assembly of collagen, by means of specific interactions with neighboring molecules in aggregates. The second problem concerns several peptides that occur in bovine pancreatic and seminal ribonucleases and have been proposed to play a role in the folding process of these molecules. The aim of both of these investigations is to establish the nature of the interactions of the peptides with the rest of the protein molecule and thereby understand their role in the folding and assembly of native proteins. Preferred conformations of the peptides will be determined by conformational energy computations, X-ray crystallography, and NMR and fluorescence measurements. Interatomic inter- actions between molecules in aggregates will be modeled by energy computations. Peptide synthesis and solution physicochemical measurements will be carried out in Scheraga's laboratory at Cornell. Crystallization and X-ray structural determination will be done by Zagari in Napoli. Conformational energy computations will be carried out by all three groups, coordinated by Nemethy at Mount Sinai. The planning of the project and the interpretation of the results will require the combination of theoretical and experimental approaches.

该奖项将支持康奈尔大学的哈罗德·舍拉加教授 大学和西奈山的乔治内梅蒂教授 医学院与教授合作研究 意大利那不勒斯大学的阿德里安娜·扎加里说。 的 合作的目的是确定 几种短肽的优选构象， 蛋白质的结构和功能重要部分。 将研究两个具体问题。 第一 问题是胶原蛋白的非螺旋端肽。 这些肽在引导和调节细胞凋亡中发挥重要作用。 通过以下方式稳定胶原的特定组装方式： 与邻近分子的特定相互作用的手段， 集料. 第二个问题涉及几种肽 出现在牛胰腺和精液核糖核酸酶中 并且已经被提出在折叠过程中发挥作用 of these molecules分子. 这两项调查的目的 是要建立的相互作用的性质 肽与蛋白质分子的其余部分，从而 了解它们在天然蛋白质折叠和组装中的作用 proteins. 肽的优选构象将通过以下来确定： 构象能量计算，X射线晶体学， 以及核磁共振和荧光测量。 原子间 聚集体中分子之间的作用将通过 能量计算 肽合成及解决方案 物理化学测量将在Scheraga的 康奈尔大学的实验室 结晶和X射线结构 扎加里将在那不勒斯做出决定。 构象 能源计算将由所有三个小组进行， 由奈梅西在西奈山协调 的规划 项目和结果的解释将需要 理论与实验相结合。