Regulation of Gene Expression by Ethylene

乙烯对基因表达的调控

• 批准号: 9004353
• 负责人: Robert Fischer
• 金额: $ 25.5万
• 依托单位: University of California-Berkeley
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1990
• 资助国家: 美国
• 起止时间: 1990-08-01 至 1993-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9004353&HistoricalAwards=false
• 关键词: Regulation; Gene; Expression; Ethylene

The long term objective of this research is to understand the molecular and cellular processes by which ethylene controls fruit ripening and gene expression. The model system is Lycopersicon esculentum (tomato). By analyzing the effect of promoter deletions on gene expression in fruit from transformed plants, and in a recently developed microprojectile transient assay system, 5'-flanking regions that control gene expression in a positive and negative manner have been identified. Sites that react with developmentally regulated DNA-binding activities have been delineated, and a clone encoding a DNA-binding protein has been isolated. The specific goal is to elucidate the mechanisms of ethylene action at the gene level. Ethylene-inducible promoter fine structure will be analyzed by determining the effect of promoter mutations on gene expression in a transient gene expression system and in transgenic fruit. Specific mutations will include deletion of sites that react with DNA binding proteins, and alterations of binding site position and orientation within the promoter. Factors that regulate ethylene inducible gene expression will be studied by cloning DNA binding proteins that bind to critical DNA regulatory sequences. The cloned gene will be used to generate specific antibodies and these tools will be used to determine the mechanism that regulates DNA binding activity. DNA binding protein function will be elucidated genetically by analyzing the phenotype of transgenic plants with reduced levels of DNA binding activity due to the expression of an antisense DNA binding protein gene. The interaction of the DNA binding protein with ethylene and/or other nuclear proteins will be analyzed. %%% The plant hormone, ethylene, controls fruit ripening. This project is an investigation, at the molecular and genetic level, of how that control is carried out.

这项研究的长期目标是了解 乙烯控制果实的分子和细胞过程 成熟和基因表达。模型系统是Lycopersicon 番茄（tomato）通过分析促进剂的作用， 来自转化植物的果实中基因表达的缺失， 在最近开发的微粒瞬时测定中， 系统中控制基因表达的5 ′-侧翼区， 积极和消极的方式已经确定。的网站 与发育调节的DNA结合活性反应， 已经描述，并且编码DNA结合蛋白的克隆已经 被孤立。具体目标是阐明机制 乙烯在基因水平上的作用。乙烯诱导型启动子 精细结构将通过确定以下因素的影响来分析： 启动子突变对瞬时基因表达的影响 表达系统和转基因果实。特定突变 将包括删除与DNA结合反应的位点 蛋白质，以及结合位点位置的改变， 启动子内的方向。调节乙烯的因素 诱导基因表达将通过克隆DNA结合来研究 与关键DNA调控序列结合的蛋白质。的 克隆的基因将用于产生特异性抗体， 这些工具将用于确定 调节DNA结合活性。DNA结合蛋白的功能将 通过分析表型， 具有降低水平的DNA结合活性的转基因植物， 反义DNA结合蛋白基因的表达。的 DNA结合蛋白与乙烯和/或其它 将分析核蛋白。 %%% 植物激素，乙烯，控制果实成熟。这 该项目是一项研究，在分子和基因水平上， 如何进行控制。