Molecular Studies on Antigen Receptor Gene Recombination

在B和T淋巴细胞的分化过程中，抗体和 T细胞抗原受体基因经历一系列复杂的 重组事件 所有的抗原受体可变区 区域基因包含一对高度保守的重组 信号序列，其是位点特异性 重组酶 为了确定和描述 在这个重组过程中，Aguilera博士 寻找核因子，这些核因子可能与 重组信号 利用灵敏的电泳技术 DNA结合因子已经在许多实验中被鉴定出来， 重组阳性前B细胞，其特异性结合至 V-（D）-J重组信号。 这项研究的下一个目标是 （1）进一步研究淋巴细胞核因子 其与重组信号特异性相互作用;（2） 为了继续进行实验， 结合因子;（3）克隆和鉴定编码 与V-（D）-J重组相互作用的核因子 信号. 本研究的长期目标是表征 参与抗原受体基因的酶机制 在免疫系统的细胞中重组。 这些事件 是产生各种各样的 受体分子识别并使个体能够 对各种外来物质产生免疫反应。

During the differentiation of B and T-lymphocytes, antibody and T-cell antigen receptor genes undergo a complicated series of recombination events. All of the antigen receptor variable region genes contain a pair of highly conserved recombination signal sequences which are the targets of a site-specific recombinase. In an attempt to identify and characterize the factors involved in this recombination process, Dr. Aguilera has sought nuclear factors which might interact specifically with the recombination signals. With the use of sensitive electrophoretic assays, DNA binding factors have been identified in recombination-positive pre-B cells which bind specifically to the V-(D)-J recombination signals. The next goals of this research are: (1) To further characterize the lymphocyte nuclear factors which interact specifically with the recombination signals; (2) To continue experiments leading to the purification of these binding factors; (3) To clone and characterize the genes encoding nuclear factors which interact with the V-(D)-J recombination signals. The long term objective of this research is the characterization of the enzymatic machinery involved in antigen receptor gene recombination in cells of the immune system. These events underlie the generation of the vast diversity of specific receptor molecules that recognize and enable an individual to develop an immune response to a wide array of foreign substances.