Molecular Studies on Antigen Receptor Gene Recombination

抗原受体基因重组的分子研究

• 批准号: 9004549
• 负责人: Renato Aguilera
• 金额: $ 41.16万
• 依托单位: University of California-Los Angeles
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1990
• 资助国家: 美国
• 起止时间: 1990-09-01 至 1995-02-28
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9004549&HistoricalAwards=false
• 关键词: Molecular; Studies; Antigen; Receptor; Gene

During the differentiation of B and T-lymphocytes, antibody and T-cell antigen receptor genes undergo a complicated series of recombination events. All of the antigen receptor variable region genes contain a pair of highly conserved recombination signal sequences which are the targets of a site-specific recombinase. In an attempt to identify and characterize the factors involved in this recombination process, Dr. Aguilera has sought nuclear factors which might interact specifically with the recombination signals. With the use of sensitive electrophoretic assays, DNA binding factors have been identified in recombination-positive pre-B cells which bind specifically to the V-(D)-J recombination signals. The next goals of this research are: (1) To further characterize the lymphocyte nuclear factors which interact specifically with the recombination signals; (2) To continue experiments leading to the purification of these binding factors; (3) To clone and characterize the genes encoding nuclear factors which interact with the V-(D)-J recombination signals. The long term objective of this research is the characterization of the enzymatic machinery involved in antigen receptor gene recombination in cells of the immune system. These events underlie the generation of the vast diversity of specific receptor molecules that recognize and enable an individual to develop an immune response to a wide array of foreign substances.

在B淋巴细胞和t淋巴细胞的分化过程中，抗体和t细胞抗原受体基因经历了一系列复杂的重组事件。所有抗原受体可变区基因都包含一对高度保守的重组信号序列，它们是位点特异性重组酶的靶点。在试图识别和描述这种重组过程中涉及的因素时，阿奎莱拉博士寻找了可能与重组信号特异性相互作用的核因素。利用敏感的电泳分析，在重组阳性的前b细胞中发现了DNA结合因子，这些结合因子特异性地结合V-(D)- j重组信号。本研究的下一个目标是：(1)进一步表征与重组信号特异性相互作用的淋巴细胞核因子；(2)继续进行纯化这些结合因子的实验；(3)克隆和鉴定与V-(D)- j重组信号相互作用的核因子编码基因。本研究的长期目标是表征免疫系统细胞中抗原受体基因重组所涉及的酶机制。这些事件是产生多种特异性受体分子的基础，这些受体分子识别并使个体能够对各种外来物质产生免疫反应。