Molecular Studies on Antigen Receptor Gene Recombination

9316981 Aguilera本研究的目的是进一步表征淋巴细胞特异性DNA重组所涉及的分子和生化机制。我们已经分离出并部分表征了核酸酶活性Endo-S，我们认为它密切参与同种型转换重组过程。我们将继续对Endo-S进行生物化学表征，对衍生自它的天然蛋白质进行测序，并产生针对它的特异性抗血清。我们将尝试克隆编码Endo-S的基因并表达它。我们还将分离和表征与它相关的任何因子。与DNA重排有关，而DNA重排是哺乳动物产生大量不同抗体的原因。也有提示性证据表明，它也可能参与体细胞DNA重排，这可能有不太理想的结果，如与癌基因相关的DNA易位。这种蛋白质的完整表征可以更好地了解其在几种不同细胞过程中的功能作用。 ***

9316981 Aguilera The goal of this research is to further characterize the molecular and biochemical mechanisms involved in lymphocyte- specific DNA recombination. We have isolated and partially characterized a nuclease activity, Endo-S, which we believe is intimately involved in the isotype switch recombination process. We will continue to biochemically characterize Endo-S, sequence the native protein derived from it and generate a specific antisera to it. We will attempt to clone the gene encoding Endo-S and express it. We will also isolate and characterize any factors associated with it. %%% It is thought that this endonuclease, Endo-S, is involved in the DNA rearrangements which account for the large numbers of different antibodies which can be made by mammals. There is also suggestive evidence that it may also be involved in somatic DNA rearrangements which could have less desirable consequences, such as DNA translocations associated with oncogenes. A complete characterization of this protein could lead to a better understanding of its functional role in several different cellular processes. ***