Molecular Studies on Antigen Receptor Gene Recombination

抗原受体基因重组的分子研究

• 批准号: 9316981
• 负责人: Renato Aguilera
• 金额: $ 35.75万
• 依托单位: University of California-Los Angeles
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1994
• 资助国家: 美国
• 起止时间: 1994-02-01 至 1998-01-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9316981&HistoricalAwards=false
• 关键词: Molecular; Studies; Antigen; Receptor; Gene

9316981 Aguilera The goal of this research is to further characterize the molecular and biochemical mechanisms involved in lymphocyte- specific DNA recombination. We have isolated and partially characterized a nuclease activity, Endo-S, which we believe is intimately involved in the isotype switch recombination process. We will continue to biochemically characterize Endo-S, sequence the native protein derived from it and generate a specific antisera to it. We will attempt to clone the gene encoding Endo-S and express it. We will also isolate and characterize any factors associated with it. %%% It is thought that this endonuclease, Endo-S, is involved in the DNA rearrangements which account for the large numbers of different antibodies which can be made by mammals. There is also suggestive evidence that it may also be involved in somatic DNA rearrangements which could have less desirable consequences, such as DNA translocations associated with oncogenes. A complete characterization of this protein could lead to a better understanding of its functional role in several different cellular processes. ***

9316981阿奎莱拉：这项研究的目标是进一步确定淋巴细胞特异性DNA重组所涉及的分子和生化机制。我们已经分离并部分鉴定了一种核酸酶活性，内切S，我们认为它与同型开关重组过程密切相关。我们将继续对内切S进行生化鉴定，对其衍生的天然蛋白进行测序，并产生针对它的特异性抗血清。我们将尝试克隆编码内切-S的基因并进行表达。我们还将分离和表征与其相关的任何因素。%据认为，这种内切酶S参与了DNA重排，这是哺乳动物产生大量不同抗体的原因。也有暗示的证据表明，它也可能参与体细胞DNA重排，这可能会产生不太令人满意的结果，例如与癌基因相关的DNA易位。对该蛋白质的完整表征可能有助于更好地理解其在几个不同细胞过程中的功能作用。***