Chromosomal Telomeres

染色体端粒

• 批准号: 9005047
• 负责人: Robert Levis
• 金额: $ 29万
• 依托单位: Fred Hutchinson Cancer Research Center
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1990
• 资助国家: 美国
• 起止时间: 1990-09-01 至 1994-02-28
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9005047&HistoricalAwards=false
• 关键词: Chromosomal; Telomeres

We are examining the telomere region of the fruitfly, Drosophila melanogaster, as a model for metazoan telomeres. We have shown that in Drosophila, in contrast to yeast, a chromosome from which the terminus has been deleted can be transmitted for many generations. Our structural analysis of the subterminal portion of the telomere region has revealed a complex interspersion of different repetitive sequences. Preliminary results suggest an unexpectedly high frequency of terminal and subterminal length variations. In addition, we have documented a case in which the expression of a gene was repressed when it was transposed to a subterminal site. The termini of eukaryotic chromosomes, called telomeres, are specialized structures thought to be required for chromosome stability. It has recently been shown that a characteristic terminal sequence motif is conserved among unicellular eukaryotes, vertebrates and higher plants. In many eukaryotes the telomere region also encompasses more complex repetitive sequences that are shared among all or a subset or the chromosome tips. Telomeres have been studied most extensively in single-celled eukaryotes and there is little known about the overall sequence organization, functions, or dynamics of the telomere region of multicellular eukaryotes.***

我们正在研究果蝇（果蝇）的端粒区域，作为后生动物端粒的模型。 我们已经证明，与酵母相反，在果蝇中，删除了末端的染色体可以遗传很多代。 我们对端粒区域亚末端部分的结构分析揭示了不同重复序列的复杂散布。 初步结果表明末端和近末端长度变化的频率出乎意料地高。 此外，我们还记录了一个案例，其中基因的表达在转座到亚末端位点时受到抑制。 真核染色体的末端称为端粒，是被认为是染色体稳定性所必需的特殊结构。 最近表明，特征性末端序列基序在单细胞真核生物、脊椎动物和高等植物中是保守的。 在许多真核生物中，端粒区域还包含在所有或子集或染色体尖端之间共享的更复杂的重复序列。 端粒在单细胞真核生物中得到了最广泛的研究，而对多细胞真核生物端粒区域的整体序列组织、功能或动态知之甚少。***