Visualization of Chromosomal Telomeres in Saccharomyces cerevisiae

酿酒酵母染色体端粒的可视化

• 批准号: 9407704
• 负责人: Judith Berman
• 金额: $ 4万
• 依托单位: University of Minnesota-Twin Cities
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1994
• 资助国家: 美国
• 起止时间: 1994-05-15 至 1996-04-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9407704&HistoricalAwards=false
• 关键词: Visualization; Chromosomal; Telomeres; Saccharomyces; cerevisiae

9407704 BERMAN The project will study the organization of telomeres in Saccharomyces cerevisiae. Fluorescence in situ hybridization experiments will be done to visualize the positions of telomere DNA and nuclear proteins which interact with this DNA. Mutant strains of the yeast with aberrant telomere structures will then be used to identify roles for individual components of the telomere complex. The investigators have already identified mutations in four different genes which impact telomere function. The principal activity supported by this award is the immuno- microscopic visualization of the telomere interactions with nuclear proteins. %%% The ends of chromosomes are termed telomeres. They have unique properties and can dramatically impact the stability of chromosomes. The DNA in a telomere is known to have a well defined, repeating pattern which facilitates its replication. Interaction of this DNA with a variety of proteins found in the nucleus of the cell is also critical for proper chromosome behavior. These interactions have not been characterized to the extent that we understand what they are and how they work. Previous work has focused on genetic and biochemical investigations of these questions. This project will extend this work to examine, at the microscopic level, what happens in living cells where telomere function has been disrupted by mutations. ***

小行星9407704 该项目将研究酿酒酵母中端粒的组织。 荧光原位杂交实验将进行可视化的端粒DNA和核蛋白的位置与此DNA的相互作用。 具有异常端粒结构的酵母突变株将用于鉴定端粒复合物的各个组分的作用。 研究人员已经确定了影响端粒功能的四种不同基因的突变。 该奖项支持的主要活动是端粒与核蛋白相互作用的免疫显微镜可视化。 染色体的末端称为端粒。 它们具有独特的性质，可以显着影响染色体的稳定性。 已知端粒中的DNA具有良好定义的重复模式，这有助于其复制。这种DNA与细胞核中发现的各种蛋白质的相互作用对于正确的染色体行为也至关重要。 这些相互作用还没有被描述到我们理解它们是什么以及它们是如何工作的程度。 以前的工作集中在这些问题的遗传和生物化学研究。 该项目将扩展这项工作，在微观水平上研究端粒功能被突变破坏的活细胞中发生了什么。 ***