Architecture and structural dynamics of core components of the translational assembly scaffold (A06)

平移组装支架核心部件的体系结构和结构动力学（A06）

• 批准号: 283706321
• 金额: --
• 依托单位: Arbeitsgruppe Zelluläre Proteostase und Massenspektrometrie
• 依托单位国家: 德国
• 项目类别: Collaborative Research Centres
• 财政年份: 2016
• 资助国家: 德国
• 起止时间: 2015-12-31 至 2022-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/283706321?language=en
• 关键词: Architecture; structural; dynamics; core; components

The central objective of this project is to better understand the structural configuration and regulation of the 60S ribosome biogenesis machinery, focusing on the DEAD-box ATPase family members involved in 60S assembly. To this end, crosslinking coupled to mass spectrometry, integrated modeling and cryo-electron microscopy will be applied to affinity purified protein complexes from cells to help elucidate the molecular function of DEAD-box proteins in the early steps of ribosome assembly. The project also aims to shift the focus from the mere structural elucidation of ribosomal assembly intermediates to a better understanding of how 60S biogenesis is actually regulated. To this end we will use structural mass spectrometry and proteomics to obtain a better picture of the occurrence and potentially also functional relevance of specific PTMs for 60S ribosome biogenesis.

该项目的中心目标是更好地了解60 S核糖体生物合成机制的结构配置和调控，重点关注参与60 S组装的DEAD-box ATP酶家族成员。为此，交联耦合质谱，集成建模和冷冻电子显微镜将应用于亲和纯化的蛋白质复合物从细胞中，以帮助阐明在核糖体组装的早期步骤中的DEAD盒蛋白的分子功能。该项目还旨在将重点从单纯的核糖体组装中间体的结构阐明转移到更好地了解60 S生物合成实际上是如何调节的。为此，我们将使用结构质谱和蛋白质组学，以获得更好的图片的发生和潜在的功能相关性的特定PTM为60 S核糖体生物合成。