Architecture and structural dynamics of core components of the translational assembly scaffold (A06)

该项目的中心目标是更好地了解60S核糖体生物发生机制的结构配置和调控，重点研究60S组装中涉及的DEAD-box atp酶家族成员。为此，交联联用质谱、集成建模和低温电镜将应用于细胞亲和纯化蛋白复合物，以帮助阐明DEAD-box蛋白在核糖体组装早期阶段的分子功能。该项目还旨在将重点从单纯的核糖体组装中间体的结构阐明转移到更好地理解60S生物发生实际上是如何调节的。为此，我们将使用结构质谱法和蛋白质组学来更好地了解特定ptm与60S核糖体生物发生的发生和潜在的功能相关性。

The central objective of this project is to better understand the structural configuration and regulation of the 60S ribosome biogenesis machinery, focusing on the DEAD-box ATPase family members involved in 60S assembly. To this end, crosslinking coupled to mass spectrometry, integrated modeling and cryo-electron microscopy will be applied to affinity purified protein complexes from cells to help elucidate the molecular function of DEAD-box proteins in the early steps of ribosome assembly. The project also aims to shift the focus from the mere structural elucidation of ribosomal assembly intermediates to a better understanding of how 60S biogenesis is actually regulated. To this end we will use structural mass spectrometry and proteomics to obtain a better picture of the occurrence and potentially also functional relevance of specific PTMs for 60S ribosome biogenesis.