Molecular Cloning of Patterning Genes in the Slime Mold Polysphondylium Pallidum

粘菌苍白多毛病基因的分子克隆

• 批准号: 9005606
• 负责人: Edward Cox
• 金额: $ 28.5万
• 依托单位: Princeton University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1990
• 资助国家: 美国
• 起止时间: 1990-10-01 至 1994-03-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9005606&HistoricalAwards=false
• 关键词: Molecular; Cloning; Patterning; Genes; Slime

Polysphondylium pallidum is a cellular slime mold that forms distinctive spatial patterns during its like cycle. One of these patterns, the radial arrangement of spore-bearing arms about a central stalk, arises from a spheroidal mass of cells at the base of the developing organism. The emergence of the radial patterning from a spheroidal one is a true symmetry breaking event which Dr. Cox proposes to study in cellular and molecular detail by examining the structure and function of the genes involved. The genes coding for patterning elements will be studied by conventional cloning techniques and used to guess at possible functions; to study the timing and regulation of their transcripts; and to use them in antisense constructs and gene disruption experiments to ask whether or not the gene products are essential for development and morphogenesis. The long range goal of this research is a complete cellular understanding of morphogenesis in this simple system, with a view to relating the various contributions made by cell-cell signaling and physical interaction, the roles played by diffusible chemical signals, and the possible contribution to the process of forces such as cell movement, surface tension, and tissue deformation.

梅毒螺旋体是一种细胞黏菌， 独特的空间模式。其中一 模式，孢子轴承臂的径向排列， 中心柄，起源于基部的球状细胞团 发育中的有机体。辐射状的出现 从一个球形的图案是一个真正的对称性破缺 考克斯博士建议在细胞和分子生物学方面研究的事件 通过检查基因的结构和功能， 涉案编码图案元素的基因 通过传统的克隆技术研究， 可能的职能;研究其时间安排和管理 转录物;并将它们用于反义构建体和基因工程中。 干扰实验来研究基因产物 是发育和形态发生所必需的远程 这项研究的目标是对细胞的完整理解， 形态发生在这个简单的系统，以期有关的 细胞-细胞信号传导和物理 相互作用，扩散化学信号所起的作用，以及 可能的贡献，如细胞的过程中， 运动、表面张力和组织变形。