An Electronic Area Detector for Protein Crystallography

这项提议要求提供资金，用于购买电子面积探测器衍射仪，用于蛋白质结构的X射线结晶学研究。这是1986年12月9日最初提交的提案的更新版本。目前正在研究的蛋白质包括大肠杆菌、鸡肉和人二氢叶酸还原酶的各种复合体和突变体，以及酵母细胞色素c过氧化物酶的突变体和复合体。此外，与DNA复制和修复相关的两种蛋白质的晶体已经结晶，并正在进行研究，以确定它们是否适合于结构确定：大肠杆菌质粒的epA1和大鼠B-聚合酶。还有其他蛋白质正在进行结晶试验。购置面积探测器衍射仪将使PI能够在晶体准备好安装时收集数据，无需等待--通常是几个月--在另一个实验室使用衍射仪；提高数据收集的速度和灵活性；允许从最少数量的晶体收集完整的数据集；并为结构改进提供准确的高分辨率数据。

This proposal requests funds for the purchase of an electronic area detector diffractometer for use in x-ray crystallographic studies of protein structures. It is an updated version of the proposal originally submitted Dec. 9, 1986. Proteins currently being investigated include a variety of complexes and mutants of E. coli, chicken and human dihydrofolate reductase, and mutants and complexes of yeast cytochrome c peroxidase. Additionally, crystals of two proteins relevant to DNA replication and repair have been crystallized and are being studied to establish their suitability for structure determination: repA1 of an E. coli plasmid and rat B-ploymerase. Still other proteins are undergoing crystallization trials. Acquisition of an area detector diffractometer will allow the PIs to collect data when crystals are ready for mounting and eliminate the need to wait, often several months, for access to a diffractometer in another laboratory; enhance the rate and flexibility of data collection; permit complete data sets to be collected from a minimum number of crystals; and provide accurate high-resolution data for structure refinement.