Solid State NMR Method Development: Protein Structure & Dynamics Elucidation

该提案描述了固体核磁共振技术的持续发展，以确定各向异性环境中的高分辨率蛋白质和多肽的结构和动力学。文中描述了脊椎扭转角的解析确定和脊椎运动的空间模型的详细描述的例子。在此描述了我们对核自旋相互作用的大小和取向的知识的进一步改进。讨论了利用新的自旋相互作用和优化利用取向约束的方法。描述了一种实现局部运动详细空间模型的新方法，并根据该空间模型对松弛数据进行了分析。最后，详细介绍了从所有光谱数据中解析结构和动态信息的统一方法。

The proposal describes the continuing development of solid state NMR techniques to determine high resolution protein and polypeptide structure and dynamics in anisotropic environments. Examples of the analytical determination of backbone torsion angles and a detailed description of a spatial model of backbone motions are described. Further refinement of our knowledge of the magnitudes and orientations of nuclear spin interactions is described herein. The utilization of new spin interactions and methods for taking optimal advantage of orientational constraints are discussed. A new approach for achieving a detailed spatial model for local motions and an analysis of relaxation data in light of the spatial model are described. Finally, a unified approach for resolving the structural and dynamic information from all of the spectroscopic data is detailed.***