Acquisition of a Peptide Synthesizer

购置肽合成仪

• 批准号: 8820672
• 负责人: Timothy Cross
• 金额: $ 4.77万
• 依托单位: Florida State University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1989
• 资助国家: 美国
• 起止时间: 1989-03-01 至 1991-02-28
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8820672&HistoricalAwards=false
• 关键词: Acquisition; Peptide; Synthesizer

The acquisition of a sophisticated peptide synthesizer for the Bioanalytical Facility at Florida State University will have a major impact on the research efforts of both chemists and biologists. For the work of Dr. Cross this capability makes possible a far more detailed structural study of membrane bound peptides by solid state NMR than has been possible before. These biophysical studies requiring large samples of isotopically labeled peptides will allow for the development of an atomic resolution picture of both the three dimensional structure and molecular dynamics. For one this capability will allow for studies of the mechanism of action of the collagenase enzymes which have a remarkable specificity not only for the amino acid sequence but also for supramolecular structural features. To deconvolute these specificity requirements, a wide range of peptides will be synthesized to more clearly define the primary sequence specificity. Futhermore it is possible to synthesize specific sequences which have certain supramolecular properties. Another requires synthetic peptides for studying the binding of antitumor agents (taxol and taxol analogs) to tublin models. While the binding studies are just being initiated this research project is getting a lot of attention and these studies are a very important step in the understanding of the taxol antitumor activity. The biologists, will also be studying synthetic segments of large proteins. One is interested in characterizing the Luteinizing Hormone Release Inhibiting Factor (LHRIF) as well as preparing analogs of super active agonists and antagonists. Another will be assaying for antibody binding to synthetic polypeptides, as well as an ability of the polypeptides to inhibit reactivity between antibody and idiotope or allotype- bearing Ig molecule.

购买一台精密的肽合成仪， 佛罗里达州立大学的生物分析机构将 对化学家和科学家的研究工作产生了重大影响， 生物学家 对于克罗斯博士的工作来说，这种能力使得 有可能进行更详细的膜结合结构研究 肽通过固态NMR比以前可能。这些 生物物理研究需要大量的同位素样品 标记的肽将允许开发一种原子 三维结构的分辨率图片， 分子动力学 首先，这种能力将允许 胶原酶作用机制的研究 它们不仅对氨基酸具有显著的特异性， 序列，而且超分子结构特征。 到 对这些具体要求进行解卷积， 肽将被合成，以更清楚地定义主要的 序列特异性 因此，可以合成 具有某些超分子性质的特定序列。 另一个需要合成肽来研究 抗肿瘤剂（紫杉醇和紫杉醇类似物）对微管蛋白模型的作用。 虽然结合研究刚刚开始， 项目得到了很多关注，这些研究是一个 这是了解紫杉醇抗肿瘤作用的重要一步 活动 生物学家也将研究合成 大蛋白质的片段。 一个是对描述 促黄体生成激素释放抑制因子（LHRIF） 用于制备超活性激动剂和拮抗剂的类似物。 另一个将是分析抗体与合成的 本发明还涉及多肽，以及所述多肽的以下能力： 抑制抗体与独特位或同种异型之间反应性- 带有IG分子。